How to implement washing and bolus injection - Data2Dynamics/d2d GitHub Wiki
Similar to the [bolus injection example](How to implement a bolus injection), sometimes a washing event should be modeled.
Such event can be modeled by an additional input function:
INPUTS
Washing C nM conc. "wash_strength * (1/sqrt(2*pi*wash_duration^2)) * exp(-(t - wash_timepoint)^2/(2*wash_duration^2))"
The function is a scaled normal distribution (given that t
indicates the independent variable) with area under the curve given by the value of wash_strength
and should be choosen large enough. The washing time point is given by wash_timepoint
and the duration by wash_duration
.
Additionally, one has to specify a reaction that removes the respective state variable (here Ligand
) proportional to the Washing
signal:
REACTIONS
Ligand -> CUSTOM "Ligand * Washing"
In the folder /Examples
we provide some showcase applications Washing_and_Injection_Test
that can be used as a starting point for a new modeling project.
In this example a first bolus of 5 nM is injected at time point 30 minutes over a period of 1 minute. At time point 50 minutes Ligand
is washed almost completely, and at time point 60 minutes another bolus of 3 nM is injected.