Tuning the Instrument - vivianlu9/EMAX_VOA_sandbox GitHub Wiki

Chemstation

  1. Check the name of the tune file you're currently using. Go to Instrument Control and click on the box labeled MS. You can select either of the highlighted options.

instrument control

Selecting `Edit MS SIM/Scan` pulls up the following screen. The tune file name is highlighted.

tune file

Selecting `Select MS Tune File` pulls up the following screen. The file name shown is the current tune file being used.

bfb screen

  1. Go to Instrument Control > Tune.
  2. Load the tune file you're currently using and save under a different name (ex: save BFB3 as BFB1).

Personally, I try to stick to using BFB, BFB1 and BFB2 because having too many BFB files gets confusing and messy to track.

  1. Go to File > Reset to defaults.
  2. Go to Tune > Maximum Sensitivity Autotune.

Do NOT retune using the original tune file unless you're not going to use it anymore. If you retune using the original tune file, it will get overwritten even if you don't save the new tune.

  1. Save the tune when it finishes. Keep the BFB printout for the tune.
  2. If needed, switch the method's tune file to the new tune and save the method.

Sometimes we retune the instrument but we don't use the new tune because the new tune may cause the responses to be drastically different from the original tune.

  1. Test the new tune to see if it works. If it works, put the BFB printout in the instrument's tune folder.

Agilent

  1. From Instrument Control, go to View > Tune and Vacuum Control.
  2. Save the current BFB tune under a different name if you want to use it for other methods.

Unlike with ChemStation, there's only one BFB tune file so retuning the instrument will always overwrite BFB.U.

  1. Select BFB Tune (BFB.U).
  2. Save the tune when it finishes. Keep the sheet that printed after retuning.
  3. Test the new tune to see if it works. If it works, put the BFB printout in the instrument's tune folder.
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