Sampling Depth

Given the expected abundance of the organism to be detected, calculate the total number of reads needed to get a specified number of on-target reads at a specified probability. A sampling depth calculator has been developed for this purposed.

You can use the calculator online here. You see the source code and download the Shiny app locally here.

Other resources: Basic calculation

Sample Processing

RNA Virus Detection

Protocol that facilitates detection of RNA viruses down to a viral load of 10^3 in human plasma samples. Adaptations for ultra-low RNA input as well as ribosomal RNA and DNA digestion are used.