There are many people posting on multiple internet forums with persistent symptoms following an exposure to various body fluids. Available clinical tests have not found an explanation. Those affected are contributing metagenomic sequencing data to facilitate a peer-to-peer investigation to attempt and find possible etiologies. If you would like to participate by contributing data, please request a kit for the appropriate sample type from Aperiomics.

Aperiomics is providing the sequencing services for this investigation. They provide a report of organisms detected with their classifier as well as raw Illumina paired-end sequencing data. The table below captures the data collected to date.

Date	Contributor	Sample Source	Sequencing Type	Forward Reads	Reverse Reads
2017-09-21	undiagnosed	Whole blood	RNA1,2	R1	R2
2017-09-21	undiagnosed	Whole blood	DNA2	R1	R2
2017-09-27	undiagnosed	Whole blood	RNA1,2	R1	R2
2017-10-12	zzz	Saliva	RNA	R1	R2
2017-11-22	undiagnosed	Plasma	RNA	R1	R2
2017-11-22	undiagnosed	Plasma	DNA	R1	R2

¹ sample RNA integrity issues, low RNA input

² too few non-human reads for Aperiomics classifier minimum confidence level

Below are reports generated by Aperiomics. Some contributors did not get raw data and only have the report. While convenient for getting a quick look, raw data is needed for this investigation.

Date	Contributor	Sample Source	Sequencing Type	Report
2017-06-20	Cheesus	Whole blood, Fecal, Urine, Oral swab	DNA	PDF
2017-10-12	zzz	Saliva	RNA	JPG
2017-11-22	undiagnosed	Plasma	RNA & DNA	PDF

While unbiased shotgun metagenomic sequencing is hypothesis free, the sampling depth must be sufficient to detect organisms at the expected abundance levels. See the Experiment Design page for more information.

Tools for analyzing and trimming reads are documented on the Quality Control page.

Various methods including metagenomics classifiers, de novo assembly, BLAST, etc. will be used during the course of this investigation. See the Methods page for more information.

If you would like to contribute to this wiki you can either create a GitHub account and request edit permissions or clone the repository and make a pull request.