participant_presentations - trinityrnaseq/BerlinTrinityWorkshop2018 GitHub Wiki
Participant Presentations
Target presentation times: anywhere from 3 minutes to 10 minutes, no longer than 10 minutes though...
Topics to include:
- Organism or system being studied
- any conditions contrasted for differential expression?
- Description of the data:
- paired-end or single-end reads
- read length
- number of biological replicates
- Quality of the read data
- did fastqc or multiqc suggest problem replicates or bad quality reads?
- Quality control of sample replicates?
- are replicates more similar to each other within samples than across samples?
- (eg. correlation plots and/or PCA plots)
- Trinity assembly stats
- total number of reads?
- number of transcripts?
- percent of reads aligned to the Trinity assembly?
- ExN50 plot? Peak ExN50 value?
- Differential expression results
- how many DE transcripts? DE genes?
- any interesting functions for DE genes?
- Anything else you'd like to highlight of interest - free reign.