participant_presentations - trinityrnaseq/BerlinTrinityWorkshop2018 GitHub Wiki

Participant Presentations

Target presentation times: anywhere from 3 minutes to 10 minutes, no longer than 10 minutes though...

Topics to include:

  • Organism or system being studied
    • any conditions contrasted for differential expression?
  • Description of the data:
    • paired-end or single-end reads
    • read length
    • number of biological replicates
  • Quality of the read data
    • did fastqc or multiqc suggest problem replicates or bad quality reads?
  • Quality control of sample replicates?
    • are replicates more similar to each other within samples than across samples?
    • (eg. correlation plots and/or PCA plots)
  • Trinity assembly stats
    • total number of reads?
    • number of transcripts?
    • percent of reads aligned to the Trinity assembly?
    • ExN50 plot? Peak ExN50 value?
  • Differential expression results
    • how many DE transcripts? DE genes?
    • any interesting functions for DE genes?
  • Anything else you'd like to highlight of interest - free reign.