Chemical Standard Operating Protocols (SOP) - sr320/LabDocs GitHub Wiki

Roberts Lab SOPs

University of Washington Lab Safety Manual

Chemical Material Safety Data Sheets (MSDS) on MyChem website.

RNAzol RT

Purpose: RNAzol RT is used for separating RNA from cells.

Specific Hazards: RNAzol RT is corrosive and harmful if inhaled.

Personal Protective Equipment:

  • REQUIRED:

    • Gloves
    • Chemical fume hood

  • WHEN SPLASH POTENTIAL EXISTS

    • Safety googles
    • Lab Coat

Waste Disposal:

  • Liquid Waste:
    • Transfer liquid waste to labeled container designated for RNAzol waste.
  • Solid Waste:
    • Tubes/tip/gloves with residual RNAzol should be stored in chemical fume hood for no less than 24hrs and then disposed in regular trash.

Prepared by: Sam White, 20161024

Isopropanol (2-propanol)

Purpose: Used in the precipitation of nucleic acids.

Specific Hazards: Highly flammable - keep away from ignition sources.

Personal Protective Equipment:

  • REQUIRED:

    • Gloves

  • WHEN SPLASH POTENTIAL EXISTS

    • Safety googles
    • Lab Coat

Waste Disposal:

  • Liquid Waste:
    • Transfer liquid waste to labeled container designated for isopropanol waste.
  • Solid Waste:
    • Tubes/tip/gloves with residual isopropanol should be stored in chemical fume hood for no less than 24hrs and then disposed in regular trash.

Prepared by: Sam White, 20161024

Diethylpyrocarbonate (DEPC)

Purpose: Used as an RNase inhibitor during handling and storage of RNA.

Specific Hazards: Skin irritant and harmful if inhaled.

Personal Protective Equipment:

  • REQUIRED:
    • Gloves
    • Chemical fume hood
  • WHEN SPLASH POTENTIAL EXISTS
    • Safety googles
    • Lab Coat

Waste Disposal:

  • Liquid Waste:
    • Transfer liquid waste to labeled container designated for DEPC waste.
  • Solid Waste:
    • Tubes/tip/gloves with residual DEPC should be stored in chemical fume hood for no less than 24hrs and then disposed in regular trash.

Prepared by: Sam White, 20161024

DNAzol

Purpose: DNAzol is used for separating DNA from cells.

Specific Hazards: DNAzol is corrosive.

Personal Protective Equipment:

  • REQUIRED:

    • Gloves

  • WHEN SPLASH POTENTIAL EXISTS

    • Safety googles
    • Lab Coat

Waste Disposal:

  • Liquid Waste:
    • Transfer liquid waste to labeled container designated for DNAzol waste.
  • Solid Waste:
    • Tubes/tip/gloves with residual DNAzol should be stored in chemical fume hood for no less than 24hrs and then disposed in regular trash.

Prepared by: Sam White, 20161024

Ethanol

Purpose: Used in the precipitation of nucleic acids.

Specific Hazards: Highly flammable - keep away from ignition sources.

Personal Protective Equipment:

  • REQUIRED:

    • Gloves

  • WHEN SPLASH POTENTIAL EXISTS

    • Safety googles
    • Lab Coat

Waste Disposal:

  • Liquid Waste:
    • Transfer liquid waste to labeled container designated for ethanol waste.
  • Solid Waste:
    • Tubes/tip/gloves with residual ethanol should be stored in chemical fume hood for no less than 24hrs and then disposed in regular trash.

Prepared by: Sam White, 20161024

Phenol:chloroform:IAA (25:24:1)

Purpose: Used for purification of nucleic acids.

Specific Hazards: Phenol:chloroform:IAA is corrosive and harmful if inhaled.

Personal Protective Equipment:

  • REQUIRED:

    • Gloves
    • Chemical fume hood

  • WHEN SPLASH POTENTIAL EXISTS

    • Safety googles
    • Lab Coat

Waste Disposal:

  • Liquid Waste:
    • Transfer liquid waste to labeled container designated for phenol:chloroform:IAA waste.
  • Solid Waste:
    • Tubes/tip/gloves with residual phenol:chloroform:IAA should be stored in chemical fume hood for no less than 24hrs and then disposed in regular trash.

Prepared by: Sam White, 20161024

Proteinase K

Purpose: Enzyme used to degrade proteins during DNA isolation.

Specific Hazards: None.

Personal Protective Equipment:

  • REQUIRED:
    • Gloves

Waste Disposal: No requirements.

Prepared by: Sam White, 20161024

Sodium acetate

Purpose: Used to precipitate nucleic acids.

Specific Hazards: Skin irritant.

Personal Protective Equipment:

  • REQUIRED:
    • Gloves

Waste Disposal:

  • Liquid Waste:
    • Dispose in sink.
  • Solid Waste:
    • Tubes/tip/gloves with residual sodium acetate can be disposed in regular trash.

Prepared by: Sam White, 20161024

Ethidium bromide (EtBr)

Purpose: Use for staining nucleic acids in agarose gels.

Specific Hazards: Mutagen and potential carcinogen.

Personal Protective Equipment:

  • REQUIRED:
    • Gloves
  • WHEN SPLASH POTENTIAL EXISTS
    • Safety googles
    • Lab Coat

Waste Disposal: NOTE: Disposal instructions are only for dilute EtBr concentrations (< 10ug/mL) that are used in the lab. Concentrations higher than this are hazardous waste and should be placed in a labeled, dedicated container.

  • Liquid Waste:
    • Dispose in sink.
  • Solid Waste:
    • Tubes/tip/gloves/gels with should be double bagged, labelled "non-hazardous", and then disposed in regular trash.

Prepared by: Sam White, 20161024

Qiagen Kits

Purpose: Use for isolation of nucleic acids.

Specific Hazards: Harmful, irritant.

NOTE: Do not combine kit reagents with bleach (sodium hypchlorite)!

Personal Protective Equipment:

  • REQUIRED:
    • Gloves
  • WHEN SPLASH POTENTIAL EXISTS
    • Safety googles
    • Lab Coat

Waste Disposal:

  • Liquid Waste:
    • Transfer liquid waste to labeled container designated for Qiagen Kit waste.
  • Solid Waste:
    • Tubes/tip/gloves with residual sodium acetate can be disposed in regular trash.

Prepared by: Sam White, 20170227

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