Histograms Window

Generating Histograms with ProteaseGuru

ProteaseGuru can generate 7 different types of Histograms based on whole proteome digestion data.

To generate a histogram, select one or more protein databases and choose a histogram type from the dropdown menu. The larger the dataset, the longer the time it will take to generate the plot.

Histograms can be exported as PDF files, and a .csv file that will contain the metadata necessary for reproducing the histograms outside of ProteaseGuru.

Histogram types:

1. Amino Acid Distribution: Distribution of amino acids for all in silico digested peptides across all proteases.
2. Peptide Length: Histogram representing the length distribution of all in silico digested peptides across all proteases.
3. Protein Sequence Coverage: Histogram representing the distribution of protein sequence coverage values for all selected proteases. Protein sequence coverage is calculated by dividing the number of amino acids belonging to peptide sequences by the length of the protein.
4. Protein Sequence Coverage (Unique Peptides Only): Histogram representing the distribution of protein sequence coverage values for all selected proteases when only unique peptides are used. Protein sequence coverage is calculated by dividing the number of amino acids belonging to peptide sequences by the length of the protein.
5. Number of Unique Peptides per Protein: Histogram representing the distribution of unique peptides across the proteins present in the selected database.
6. Predicted Peptide Hydrophobicity: Histogram representing the distribution of predicted peptide hydrophobicity values.
7. Predicted Peptide Electrophoretic Mobility: Histogram representing the distribution of predicted peptide electrophoretic mobility values.