ProteaseGuru: Free, Open-Source In Silico Digestion

Welcome to the ProteaseGuru wiki!

ProteaseGuru is a in silico digestion tool to aid in bottom-up experimental planning. ProteaseGuru accepts 1+ protein databases ( .xml or .fasta) as input, and allows users to set digestion conditions and compare proteases. ProteaseGuru is designed to help users determine which protease or combination of proteases would yield the best experimental results.

ProteaseGuru provides:

• The ability to analyze more than one database - enabling its use with multi-species samples.
• Result files containing various biophysical properties of theoretical peptides such as their molecular weight, hydrophobicity and electrophoretic mobility.
• Determination of the 'uniqueness' of a theoretical sequence (both in a single database and across all databases used for analysis).
• Histograms of whole proteome proteolytic digests for the direct comparison of proteases.
• Protein sequence coverage maps for visualization of the in silico digestion of specific proteins of interest.

What is a Wiki?

ProteaseGuru's Wiki is a resource available to you to help explain the inner working of the tool. The documentation of the Wiki will gradually expand as does ProteaseGuru. Additionally, feel free to browse the codebase itself if you are familiar with C#!

What if I have a suggestion or need more help?

If you have an issue, question, or have encountered a problem with the program there are two main ways to communicate with the ProteaseGuru team. First, you can use the "Issues" tab of this GitHub, and make a new issue based on your concern. Second, you can email us a [email protected], which is a more private way of communicating with us. Either method is fine, and we will do our best to be as responsive as possible!