Check list - sequana/fastqc GitHub Wiki
Before launching the pipeline
When you prepare the pipeline, a few information are printed on the screen. Check for:
- the number of samples (should be >0)
- check the content of the script fastqc.sh, especially on a SLURM cluster to check the slurm options.
After the pipeline has finished:
- check that the file summary.html exists. If not, it means something went wrong in creating the HTML file. If so, check whether the file multiqc/multiqc_report.html exists
- Open the file summary.html. This is were your expertise will now be required. To help you deciphering the fastqc and multiqc reports, you should look at the fastqc manual: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/ to get help.
You may also find convenient to visit the drylab page of the biomics platform: https://biomics.pasteur.fr/drylab/ to get more help. In particular, please look at this PDF document: https://biomics.pasteur.fr/drylab/of_demutiplexing_and_fastqc.pdf