Conventions - phonchi/ASCEP GitHub Wiki
Contrast
White on Black - Relion, Xmipp, EMAN2, CryoSparc (Cryo-EM data is typically recorded as Black on White but will invert during processing)
Black on White - Frealign (CisTEM)
Pixel size
For the format define in RELION the actual pixel size is rlnDetectorPixelSize * 10000 / rlnMagnification