Home - mesoSPIM/mesoSPIM-hardware-documentation GitHub Wiki

Welcome to the mesoSPIM wiki! This wiki serves as documentation on how to set up, align and operate a mesoSPIM.

What is the mesoSPIM "Mainframe"?

The mesoSPIM (mesoscale selective plane illumination microscopy) project aims at creating open-hardware microscopy platforms for imaging in cleared tissue. Core features of the current generation mesoSPIM setups are:

• compatibilty with all major clearing techniques including CLARITY, CUBIC, and iDISCO
• large field-of-views, ranging from 2 to 20 mm using an Olympus MVX-10 macroscope
• dual-sided excitation
• optimized for fast screening of whole mouse brain samples, yielding relatively small datasets (10-14 GB/brain/color)
• near-isotropic sampling (6 µm in X,Y & Z) due to an axially swept lightsheet which leads to uniform z-resolution across the field-of-view
• modular sample holders and quick sample exchange
• large travel range (45 x 45 x 100 mm) to allow imaging of very large samples

Inspired by the openSPIM and openSPIN projects, the documentation for building and extending your own mainframe mesoSPIM is available here.