SB Isoelectric point - mendessoares/BuddySuite GitHub Wiki

--isoelectric_point, -ip

Description

Returns a list of isoelectric points for each sequence in the file. If passing in nucleic acid sequences, they will be translated first.

Example

Input file: Mnemiopsis_pep.fa

>Mle-Panxα12 cDNA - ML25997a.
MVIDILSGFKGITPFKGITLDDGWDQINRSFMFVLCVLMGTVVTVRQYAGGIISCDGFTK
YSGSFSEDYCWTQGLYTIKEAYDLLTMNVPYPGVIPEDMPTCIERELINGGRVSCPDPET
VKPPTRVYHLWYQWVPFYFWLAAAAFFFPYLIYKHFGVGDLKPLIQMLHNPIVDEGDQNC
MAEKASMWLFYKLNVFMNENTIFAILTEKHRLFFIVMLVKVLYLIISILALYLTDEMFHI
GSFVSYGSEWATSLPEGDNETTLVKDKLFPKMVACEIKRWGPTGLEEEQGMCVLAPNVIN
QYLFLILWFAIIFCIACNCLSVLFALTKLVFVLGSYKRLLASAFLKDELHYKHMFFNIGT
SGRVLLQIVATNVSPRVFESIMANLATKLIAERLKGNGKGSV*
>Mle-Panxα9 cDNA - ML47742a.
MLDILSKFKGVTPFKGITIDDGWDQLNRSFMFVLLVVMGTTVTVRQYTGSVISCDGFKKF
GSTFAEDYCWTQGLYTVLEGYDQPSQNIPYPGLLPDEAPPCTPVRLKDGTRLKCPDPDQL
LSPTRISHLWYQWVPFYFWLAAAAFFMPYLLYKNFGMGDIKPLVRLLHNPVESDQELKKM
TDKAATWLFYKFDLYMSEQSLLASLTRKHGLGLSMVFVKILYAAVSFGCFLLTAEMFSIG
DFKTYGSEWIKKLKLEDNLATEEKDKLFPKMVACEVKRWGASGIEEEQGMCVLAPNVINQ
YLFLILWFCLVFVMFCNIVSIFASLIKLLFTYGSYRRLLSTAFLRDDSAIKHMYFNVGSS
GRLILHVLANNTAPRVFEDILLTLAPKLIQRKLRGNGKAL*
>Mle-Panxα10B cDNA - ML32831a-2.
MRLSEKSTSHDCKACITRSHNEDCARRWGITIDDGWDQLNRSFMFGLLVVMGTTVTVRQY
TGSVISCDGFKKFGSTFAEDYCWTQGQYTVLEGYDQPNQNIPCPVPRPPSRRGSTLNTMS
QTQGFLHNPVESDQELKKMTDKAATWLFYKFDLYMSEQSLLASLTNKHGLGLSVVFVKIL
YAAVSFGCFLLTADMFSIGDFKTYGSEWINKLKLEDNLATEEKDKLFPKMVACEVKRWGA
SGIEEEQGMCVLAPNVINQYLFLILWFCLVFVMFCNIVSIFASLIKLLFTYGSYRRLLST
AFLRDDSAIKHMYFNVGSSGRLILHVLANNTAPRVFEDILLTLAPKLIQRKLRAKDYD*

Usage

$: sb Mnemiopsis_pep.fa -ip

Output

ID			   pI
Mle-Panxα12	6.011
Mle-Panxα9		8.686
Mle-Panxα10B	8.21