Introduction

The HPC-T-Annotator tool is an efficient solution for parallelizing homology annotation software, significantly reducing waiting times and enabling the processing of large transcriptomes, particularly beneficial for eukaryotic ones with substantial data. It's revealed that transcriptome size, rather than the number of sequences, mainly affects execution time, and partitioning the transcriptome into manageable sequence files enhances computational efficiency and parallelism. This tool reduces execution and waiting times from days to hours or even minutes, offering a user-friendly graphical interface and command-line execution options. It's adaptable for concurrent annotation of multiple transcriptomes across diverse databases, enhancing the analysis of extensive 'omics datasets. Moreover, its scalable and efficient framework makes it suitable for parallelizing various bioinformatics tasks, potentially advancing research in the field. Future IT adaptations are planned to expand its compatibility to clusters with schedulers other than SLURM, further broadening its user base.