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Yeast two hybrid

  • Yeast strain A (leu, trp, his, ade)

    • vector with
      • DB+Protein_1
      • LEU

  • Yeast strain B (leu, trp, his, ade)

    • vector with
      • AD + CYH2 + Protein_2
      • TRP

  • Mating

    • (DB + protein_1) + (protein_2 + AD + CYH2) result in HIS activation and survival in Sc-His, CHX sensitivity
    • LEU + TRP result in survival in Sc-Leu-Trp shows successful transformation and mating.
    • Survival with CHX treatment means no AD + CYH2 existence (Autoactivation)