8. Calculating the Final Bath Concentration (Organ Bath) - johndempster/OBSIM GitHub Wiki

When adding drugs to the organ bath, the final bath concentration (FBC), in M (Moles/litre)., is related to the stock solution concentration [stock] (in M) the volume of stock added to the bath (in ml) and the bath volume (ml) by the formula.

[FBC] = ([stock] x Volume added) / Bath volume

To determine the volume to add to achieve a specified FBC:

a) Select the Dilution Formula page and Volume to Add as the Result.

b) Enter the bath volume (10 ml) into the Bath Volume box.

c) Enter the required FBC in the Final Bath Conc box.

d) Enter the selected stock solution concentration in the Stock Soln. Conc. box.

e) Click the Calculate button.