7. Adding an Unknown Drug - johndempster/OBSIM GitHub Wiki

To add an unknown drug drug to the organ bath or Krebs’ solution reservoir:

a) Select the Unknowns page.

b) Select the drug to be applied from the list of available unknown drugs.

c) Select the concentration of the drug solution to be applied from the Stock Soln. list.

d) Enter the volume (between 0 and 1 ml) of the stock solution to be applied into the Volume box.

e) Select Organ Bath from the Add to list if the drug is to be applied directly to the organ bath. Select Reservoir if the drug is to be added to the Krebs solution reservoir.

f) Click the Add to button to inject the volume of the selected stock solution agonist into the organ bath or reservoir.