Miniprep and PCR cleanup buffers - glasgowlab/home GitHub Wiki
PCR purification buffer
- 5.5 M GuHCL
- 20 mM Tris pH 6.6
P1 buffer
- 50 mM Tris pH 8.0
- 10 mM EDTA pH 8.0
- 100 ug/mL RNase A (then refrigerate)
P2 lysis buffer
- 0.2 M NaOH
- 1% SDS
N3 buffer
- 4 M GuHCL
- 0.5 M K-Acetate
- adjust to pH 4.2
PE buffer
- 80% EtOH
- 2 mM Tris pH 7.5
- 20 mM NaCl