Miniprep and PCR cleanup buffers - glasgowlab/home GitHub Wiki

PCR purification buffer

• 5.5 M GuHCL
• 20 mM Tris pH 6.6

P1 buffer

• 50 mM Tris pH 8.0
• 10 mM EDTA pH 8.0
• 100 ug/mL RNase A (then refrigerate)

P2 lysis buffer

• 0.2 M NaOH
• 1% SDS

N3 buffer

• 4 M GuHCL
• 0.5 M K-Acetate
• adjust to pH 4.2

PE buffer

• 80% EtOH
• 2 mM Tris pH 7.5
• 20 mM NaCl