The Mystery of AAV‐5 Origins - giffordlabcvr/Parvovirus-GLUE GitHub Wiki

Background

Originally discovered as contaminants of adenovirus preparations, and despite decades of research using tissue culture models, the natural biology of AAVs remains largely unknown. Current gene therapy applications predominantly focus on AAV isolates derived from viruses that circulate in humans and non-human primates. However, one historically significant AAV isolate, AAV-5, appears to be of ungulate origin.

Importantly, despite its non-primate origin, recombinant AAV-5 vectors have been successfully produced, demonstrating the versatility of ‘trans-encapsidating’ AAV-2 vector genomes with heterologous AAV capsids.

Hypothesis of Origin

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Schematic diagram showing possible modes through which AAV-5 infections of humans might occur.

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