Category |
FIELD |
KEYWORD |
DESCRIPTION |
TYPE |
Example |
QUERY |
Query DATA |
LOCATION |
CHR |
#CHR |
Chromosome |
String |
‘1’ |
#CHR==’1’ |
|
|
POS |
#POS |
Position |
Interger |
69063 |
#POS==69063 |
|
|
|
#CHRPOS |
Chromosome:Position |
String |
‘1:69063’ |
#CHRPOS== ‘1:69063’ |
|
GENE |
GENE |
#GENE |
Gene Name |
String |
|
data[#Gene]=== true |
{“BRCA1”:true,”BRCA2”:true,”TNNC1”:true} |
GENOMIC AND GENETIC DATA |
REF |
#REF |
Reference Allele |
String |
‘A’ |
#REF==’A’ |
|
|
ALT |
#ALT |
Alternate Allele |
String |
‘T’ |
#ALT==’T’ |
|
|
AA |
#AA |
Amino Acid |
String |
‘M289T’ |
#AA==’M289T’ |
|
|
HGVS |
#HGVS |
Representation of the variant in HGVS (Human Genome Variation Society) nomenclature |
String |
‘c.6390C>T / p.Gly2130Gly’ |
#HGVS==’c.6390C>T / p.Gly2130Gly’ |
|
|
ZYG |
#GT |
Genotype Proband |
String |
‘HET’, ‘HOM’, ‘HEM’,’REF’ |
#GT==’HET’ |
|
|
|
#FATHER_GT |
Genotype of Father |
String |
‘HET’, ‘HOM’, ‘HEM’,’REF’ |
#FATHER_GT==’HET’ |
|
|
|
#MOTHER_GT |
Genotype of Mother |
String |
‘HET’, ‘HOM’, ‘HEM’,’REF’ |
#MOTHER_GT==’HET’ |
|
|
REFSEQ |
#REFSEQ |
The transcript ID(s) relevant to the effect on the protein. This track contains RefSeq Gene transcripts annotated by the NCBI Homo sapiens Annotation Release. |
String |
‘NM_001458.5’ |
#REFSEQ==’NM_001385640.1’ |
|
|
EXON # |
#EXON |
Exon # – The impacted exon of the gene |
integer |
1 |
N/A |
|
|
CODON |
#CODON |
Codon change |
String |
‘ggC/ggT’ |
#CODON==’ggC/ggT’ |
|
|
DBSNP |
#RS_NUMBER |
Known identifier (dbSNP RSID) |
String |
‘rs746751083’ |
#RS_NUMBER==’rs746751083’ |
|
|
DBSNP VERSION |
#DBVER |
The earliest dbSNP version which included the variant |
Interger |
144 |
#DBVER>144 |
|
|
RMSK |
#RMSK |
Percentage of bases overlapping genomic repeats |
Float (0-100) |
10 |
#RMSK>10 |
|
|
PAF |
#PAF |
Phenotype Allele Frequency |
Float |
10 |
#PAF>10 |
|
|
MOTHER_AFF |
#MOTHER_AFF |
Affection status in Mother |
String |
‘Unkown’,’Affected’,’Unaffected’ |
#MOTHER_AFF==’Affected’ |
|
|
FATHER_AFF |
#FATHER_AFF |
Affection status in Father |
String |
‘Unkown’,’Affected’,’Unaffected’ |
#FATHER_AFF==’Affected’ |
|
ACMG |
ACMG |
#ACMG |
ACMG Classification |
String |
‘Pathogenic’, ‘Likely Pathogenic’, ‘Variant of Unknown Significance’, ‘Likely Benign’, ‘Benign’ |
#ACMG==’Pathogenic’ |
|
|
|
#ACMGLEVEL |
The ACMG class level in number |
integer |
“Benign’: 1,’Likely benign’: 2,’Uncertain significance’: 3,’Uncertain-significance’: -3,’Likely pathogenic’: 4,’Pathogenic’: 5,’0’: 0” |
#ACMGLEVEL==1 |
|
|
DOM/REC |
#ACMG_REC_DOM_LEVEL |
The MAX acmg level between REC/DOM - for fast track only |
integer |
1 |
#ACMG_REC_DOM_LEVEL>1 |
|
|
INT_DOMAIN |
#INT_DOMAIN |
Interger Domain |
integer |
1 |
#INT_DOMAIN>1 |
|
VARIANT CALLING Q&R |
Q&R |
#QR_Score |
“Classifies calls by quality. Low = coverage < 10x and GQ < 15; Med = coverage < 20x and GQ < 50; High = coverage >20x and GQ = 50.” |
String |
‘High’, ‘Med’, ‘Low’ |
#QR_Score==’High’ |
|
DEPTH |
#DEPTH |
Read depth (total reads) |
Interger |
50 |
#DEPTH>50 |
|
|
DP2 |
#DP2 |
Read Depth (Ref/Alt) |
String |
‘12,24’ |
#DP2==’12,24’ |
|
|
%ALT |
#ALT_PERCENT |
The percentage of reads showing the alternative allele |
Interger |
48.50 |
#ALT_PERCENT>48.50 |
|
|
GQ |
#GQ |
Genotype Quality |
Interger |
50 |
#GQ>50 |
|
|
FILTER |
#FILTER |
Quality filter status based on the variant caller |
String |
‘PASS’ |
#FILTER==’PASS’ |
|
|
PL |
N/A |
Phred-scaled genotype likelihoods (HOM REF, HET, HOM ALT) |
N/A |
N/A |
N/A |
|
|
AMP |
N/A |
Amplification score (coverage/median coverage) |
N/A |
N/A |
N/A |
|
CLINICAL EVIDENCE |
PHENO |
#PHENO |
Phenotype score |
float |
N/A |
N/A |
|
|
MATCHED COUNT |
#MATCHED_PHENO_CNT |
Number of matched phenotypes |
integer |
N/A |
N/A |
|
|
MATCHED |
#MATCHED_PHENO |
The matched phenotypes |
array |
[‘brain’,’bone’] |
N/A |
|
|
CLINVAR |
#CV_CLINSIG |
The ClinVar database from NCBI |
String |
‘Pathogenic’, ‘Likely Pathogenic’, ‘Variant of Unknown Significance’, ‘Likely Benign’, ‘Benign’ |
#CV_CLINSIG==’Pathogenic’ |
|
|
OMIM |
#OMIM_GENE |
OMIM © is a comprehensive, authoritative compendium of human genes and genetic phenotypes that is freely available and updated daily. |
Boolean |
‘BRCA1’ |
data[#OMIM_GENE]=== true |
{“BRCA1”:true,”BRCA2”:true,”TNNC1”:true} |
|
OMIM INHERITANCE |
#OMIM_GENE_ABBR |
OMIM © is a comprehensive, authoritative compendium of human genes and genetic phenotypes that is freely available and updated daily. OMIM is authored and edited at the McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, under the direction of Dr. Ada Hamosh. Its official home is omim.org. This track displays inheritance information. Includes: Autosomal Dominant (AD), Autosomal Recessive (AR), Multifactorial (MG), Somatic Mutation (SO). |
string |
N/A |
N/A |
|
|
CIVIC |
N/A |
N/A |
N/A |
N/A |
N/A |
|
|
PUBS |
#MM |
MasterMind Document Count |
Interger |
11 |
#MM>11 |
|
IN HOUSE |
VARIANT |
N/A |
N/A |
N/A |
N/A |
N/A |
|
|
GENE |
N/A |
N/A |
N/A |
N/A |
N/A |
|
|
AF (%) |
#LOCAL_AF |
The allele frequency of the variant in all samples of the curreThe allele frequency of the variant in all the samples of the current account. |
Interger |
0 |
#LOCAL_AF>10 |
|
|
#LOCAL HET |
#LOCAL_HET |
The count of samples with this heterozygous variant in this account. |
Interger |
0 |
#LOCAL_HET>0 |
|
|
#LOCAL HOM |
#LOCAL_HOM |
The count of samples with this homozygous variant in this account. |
Interger |
0 |
#LOCAL_HOM>0 |
|
|
#LOCAL HEM |
#LOCAL_HEM |
N/A |
N/A |
N/A |
N/A |
|
EFFECT & PREDICTION |
EFFECT |
#EFFECT |
The effect of the variant on the protein (including splicing effects) |
String |
‘Downstream’,’Exon’,’Exon, Splice Site region’,’Frameshift’,’Frameshift, Intron, Splice site donor’, ‘Splice site region’,’Frameshift, Nonsense’,’Frameshift, Splice site’,’Frameshift, Stop readthrough’,’Inframe indel’,’Inframe indel, Splice site region’,’Intergenic’,’Intron’,’Intron, Splice site acceptor’,’Intron, Splice site donor’,’Intron, Splice site region’,’Missense’,’Missense + Inframe indel’,’Missense, Splice site region’,’Nonsense’,’Synonymous’,’Splice site region’,’Splice site region, nonsynonymous’,’Splice site region, synonymous’,’Start gained’,’Start gained, UTR’,’Start lost’,’Stop change’,’Stop readthrough’,’UTR_3_Prime’,’UTR_5_Prime’,’Upstream’,” |
#EFFECT==’Missense’ |
|
|
SEV |
#SEVERITY |
Severity (High, Med, Low) of the impact on the protein based on the Effect and, in case of missense, the predicted damage to the protein. |
String |
‘Low’, ‘Med’, ‘High’ |
#SEVERITY==’High’ |
|
|
CADD(PHRED) |
#CADD_PRED |
Combined Annotation Dependent Depletion (CADD) is a tool for scoring the deleteriousness of single nucleotide variants as well as insertion/deletions variants in the human genome. CADD Scores are computed for all SNVs and ~20 million known insertions/deletions.PHRED-scaled scores (“scaled C-scores”) ranging from 1 to 99, based on the rank of each variant relative to all possible 8.6 billion substitutions in the human reference genome. For example, reference genome single nucleotide variants at the 10th-% of CADD scores are assigned a PHRED Score of 10, top 1% to PHRED-20, top 0.1% to PHRED-30, etc. |
N/A |
N/A |
N/A |
|
|
CADD(RAW) |
#CADD_RAW |
Raw CADD scores, or “C-scores”, come straight from the Support Vector Machine model. These values have no absolute unit of meaning. However, raw values do have relative meaning, with higher values indicating that a variant is more likely to be simulated (or “not observed”) and therefore more likely to have deleterious effects. |
N/A |
N/A |
N/A |
|
|
REVEL |
#REVEL_SCORE |
Rare Exome Variant Ensemble Learner (REVEL) is an ensembl method for predicting the pathogenicity of missense variants based on a combination of scores from 13 individual tools: MutPred, FATHMM v2.3, VEST 3.0, PolyPhen-2, SIFT, PROVEAN, MutationAssessor, MutationTaster, LRT, GERP++, SiPhy, phyloP, and phastCons. |
Interger |
10 |
#REVEL_SCORE>0.1 |
|
|
SPLICE-AI |
#SAIS |
SpliceAI is a deep neural network that accurately predicts splice junctions from an arbitrary pre-mRNA transcript sequence, enabling precise prediction of noncoding genetic variants that cause cryptic splicing. In the paper, Jaganathan et al 2019, a detailed characterization is provided for 0.2 (high recall), 0.5 (recommended), and 0.8 (high precision) cutoffs. |
N/A |
N/A |
N/A |
|
|
PHYLOP |
#PHYLOP |
PhyloP (phylogenetic p-values) conservation score based on the multiple alignments of 20 mammalian genomes (including human). The larger the score, the more conserved the site. Scores range from -20.0 to 10.003.easures evolutionary conservation at individual alignment sites |
N/A |
N/A |
N/A |
|
|
GERP NR |
#GERP_NR |
Genomic Evolutionary Rate Profiling Neutral Rate (GERP NR) is a method for producing position-specific estimates of evolutionary constraint using maximum likelihood evolutionary rate estimation. |
Integer |
1 |
#GERP_NR>1 |
|
|
GERP RS |
#GERP_RS |
Genomic Evolutionary Rate Profiling Rejected Substitutions (GERP NR) is a method for producing position-specific estimates of evolutionary constraint using maximum likelihood evolutionary rate estimation. |
Interger |
1 |
#GERP_RS>1 |
|
|
LRT PRED |
#LRT_PRED |
Likelihood Ratio Test (LRT) prediction, D(eleterious), N(eutral) or U(nknown). |
String |
‘D’,’N’,’U’ |
#LRT_PRED==’D’ |
|
|
MUTTASTER |
#MUTATIONTASTER_PRED |
MutationTaster prediction, “A” (“disease_causing_automatic”), “D” (“disease_causing”), “N” (“polymorphism, [probably harmless]”) or “P” (“polymorphism_automatic, [known to be harmless]”). |
String |
‘D’ |
#MUTATIONTASTER_PRED==’D’ |
|
|
POLYPHEN2HDIV |
#POLYPHEN2_HDIV_PRED |
Polyphen2 prediction based on HumDiv, “D” (“probably damaging”), “P” (“possibly damaging”), and “B” (“benign”). |
String |
‘D’ |
#POLYPHEN2_HDIV_PRED==’D’ |
|
|
POLYPHEN2HVAR |
#POLYPHEN2_HVAR_PRED |
Polyphen2 prediction based on HumVar, “D” (“probably damaging”), “P” (“possibly damaging”) and “B” (“benign”). |
String |
‘D’ |
#POLYPHEN2_HVAR_PRED==’D’ |
|
|
SIFT |
#SIFT_SCORE |
Sort intolerated from tolerated (SIFT) scores range from 0 to 1. The smaller the score the more likely the SNP has damaging effect. Deleterious (sift<=0.05); tolerated (sift>0.05). |
Integer |
0 |
#SIFT_SCORE>0 |
|
UNIPROT |
#UNIPROT_ACC |
The Universal Protein Resource (UniProt) is a comprehensive resource for protein sequence and annotation data. The entry is the accession of the protein. |
String |
‘P07602’ |
“#UNIPROT_ACC==’P07602’ |
|
|
ADA SCORE |
#ADA_SCORE |
Ensembl prediction score based on ada-boost. Ranges 0 to 1. The larger the score the higher probability the scSNV will affect splicing. The suggested cutoff for a binary prediction (affecting splicing vs. not affecting splicing) is 0.6. |
Integer |
0.1 |
#ADA_SCORE>0.1 |
|
|
RF SCORE |
#RF_SCORE |
Ensembl prediction score based on random forests. Ranges 0 to 1. The larger the score the higher probability the scSNV will affect splicing. The suggested cutoff for a binary prediction (affecting splicing vs. not affecting splicing) is 0.6. |
Interger |
0.5 |
#RF_SCORE>0.5” |
|
|
LEN |
#SV_LEN |
The CNV/SV length |
Interger |
123 |
#SV_LEN>123 |
|
|
%RMSK |
#SV_RSML_PERCENT |
The percentage of bases overlapping genomic repeats |
Float (0-100) |
10 |
#SV_RSML_PERCENT>10 |
|
|
M.EXONS |
#SV_EXONS |
Maximum nuber of exons affected within genes by this CNV |
Interger |
10 |
#SV_EXONS>10 |
|
|
ENHS |
#SV_ENHANCERS |
Number of enhancers affected by this CNV |
Interger |
10 |
#SV_ENHANCERS>1 |
|
|
GENES |
#SV_GENES |
Number of genes affected by this CNV |
Interger |
10 |
#SV_GENES>1 |
|
|
MAX AF (GAIN) |
#SV_MAX_AF_GAIN |
Maximum allele frequency for duplications in reference populations |
Interger (<100) |
10 |
#SV_MAX_AF_GAIN <10 |
|
|
MAX AF (LOSS) |
#SV_MAX_AF_LOSS |
Maximum allele frequency for deletions in reference populations |
Interger (<100) |
10 |
#SV_MAX_AF_LOSS <10 |
|
FREQUENCY |
MAX AF(%) |
#MAX_AF |
Maximum observed allele frequency in 1000 Genomes, ESP and gnomAD |
Interger |
1 |
#MAX_AF>1 |
|
|
#HOM |
#HOM_TOTAL |
The count of samples with this homozygous allele in gnomAD |
Interger |
10 |
#HOM_TOTAL<10 |
|
|
#HET |
#HET_TOTAL |
The count of samples with this heterozygous allele in gnomAD |
Interger |
2 |
N/A |
|
|
#HEM |
#HEM_TOTAL |
The count of samples with this hemizygous allele in gnomAD |
Interger |
3 |
#HEM_TOTAL>3 |
|
|
MitoMap AF (%) |
#MM_AF |
The allele frequency of the variant in MitoMap |
Float (0-100) |
.2 |
#MM_AF>.2 |
|
|
Internal Allele Frequency in WGS(%) |
#INT_WGS_AF |
Internal allele frequency of this variant in WGS |
Float (0-100) |
.2 |
#INT_WGS_AF>.2 |
|
|
Internal Allele Frequency in WES(%) |
#INT_WES_AF |
Internal allele frequency of this variant in WES |
Float (0-100) |
.2 |
#INT_WES_AF>.2 |
|
|
1K GENOME AF (%) |
#1000GP1_AF |
The allele frequency of the variant in 1000 Genome |
Interger |
.2 |
#1000GP1_AF>.2 |
|
|
ESP AFRICAN AF (%) |
#ESP6500_AA_AF |
The allele frequency of the variant in ESP (Exome Sequencing Project) African Americans population |
Interger |
.3 |
#ESP6500_AA_AF>.3 |
|
|
ESP EUROPEAN AF (%) |
#ESP6500_EA_AF |
The allele frequency of the variant in ESP (Exome Sequencing Project) European population |
Interger |
.4 |
#ESP6500_EA_AF>.4 |
|
|
DBSNP AF (%) |
#DBGMAF |
The minor allele frequency of the variant in dbSNP |
Interger |
.5 |
#DBGMAF>.5 |
|
|
HAN AF (%) |
#CVR_AF |
The allele frequency of the variant in CONVERGE project for Han Chinese population |
Interger |
.6 |
N/A |
|
|
GNE AF (%) |
#GNE_AF |
The gnomAD Exomes allele frequency |
Interger |
.7 |
#GNE_AF>.7 |
|
|
GNG AF (%) |
#GNG_AF |
The gnomAD Genomes allele frequency |
Interger |
.8 |
#GNG_AF>.8 |
|
|
GNE AMR |
#GNE_AF_AMR |
The gnomAD Exomes Latino/Admixed American allele frequency |
Interger |
.9 |
#GNE_AF_AMR>.9 |
|
|
GNG AMR |
#GNG_AF_AMR |
The gnomAD Genomes Latino/Admixed American allele frequency |
Interger |
.10 |
#GNG_AF_AMR>.1 |
|
|
GNE AFR |
#GNE_AF_AFR |
The gnomAD Exomes African/African American allele frequency |
Interger |
.11 |
#GNE_AF_AFR>.11 |
|
|
GNG AFR |
#GNG_AF_AFR |
The gnomAD Genomes African/African American allele frequency |
Interger |
.12 |
#GNG_AF_AFR>.12 |
|
|
GNE ASJ |
#GNE_AF_ASJ |
The gnomAD Exomes Ashkenazi Jewish allele frequency |
Interger |
.13 |
#GNE_AF_ASJ>.13 |
|
|
GNG ASJ |
#GNG_AF_ASJ |
The gnomAD Genomes Ashkenazi Jewish allele frequency |
Interger |
.14 |
#GNG_AF_ASJ>.14 |
|
|
GNE EAS |
#GNE_AF_EAS |
The gnomAD Exomes East Asian allele frequency |
Interger |
.15 |
#GNE_AF_EAS>.15 |
|
|
GNG EAS |
#GNG_AF_EAS |
The gnomAD Genomes East Asian allele frequency |
Interger |
.16 |
#GNG_AF_EAS>.16 |
|
|
GNE FIN |
#GNE_AF_FIN |
The gnomAD Exomes European (Finnish) allele frequency |
Interger |
.17 |
#GNE_AF_FIN>.17 |
|
|
GNG FIN |
#GNG_AF_FIN |
The gnomAD Genomes European (Finnish) allele frequency |
Interger |
.18 |
#GNG_AF_FIN>.18 |
|
|
GNE NFE |
#GNE_AF_NFE |
The gnomAD Exomes European (non-Finnish) allele frequency |
Interger |
.19 |
#GNE_AF_NFE>.19 |
|
|
GNG NFE |
#GNG_AF_NFE |
The gnomAD Genomes European (non-Finnish) allele frequency |
Interger |
.20 |
#GNG_AF_NFE>.20 |
|
|
GNE OTH |
#GNE_AF_OTH |
The gnomAD Exomes Other allele frequency |
Interger |
.21 |
#GNE_AF_OTH>.21 |
|
|
GNE SAS |
#GNG_AF_SAS |
The gnomAD Exomes South Asian allele frequency |
Interger |
.22 |
#GNG_AF_SAS>.22 |
|