CryoEM Facility Micoscopes

Talos Arctica

Parameter	Value
Voltage	200,000 V
Cs	1.7

Magnification	Å/pix	FOV (micons)
240,000	0.41	0.17
190,000*	0.52	0.21
120,000*	0.84	0.34
92,000*	1.14	0.47
73,000*	1.35	0.55
57,000	1.789	0.74
45,000	2.254	0.94
36,000	2.853	1.15
28,000	3.668	1.51
22,000	4.649	1.98
17,500	5.961	2.58
13,500	7.532	3.24
11,000	9.505	4.08
8,500	12.32	5.29
6,700	15.67	6.67
5,300	18.86	8.11

Pixelsizes are calculated in SerialEM and could differ from EPU and Tomography Metadata.

(*) = 3D refined pixelsize using Apoferritin reconstructions fitted in X-ray structure.

Data Sheet - Falcon Detector

JEM 1400 Flash

Parameter	Value
Voltage	120,000 V
Cs	3.4
AmpC	0.7

Magnification	Å/pix
2000	34.15
2500	26.83
3,000	22.7
4,000	16.9
5,000	13.5
6,000	11.41
8,000	8.618
10,000	6.72
12,000	5.701
15,000	4.466
20,000	3.377
25,000	2.735
30,000	2.203
40,000	1.667
50,000	1.344
60,000	1.109
80,000	0.826
100,000	0.658
120,000	0.548