Operation manual - dogerber/temperature_gradient_microscopy_stage Wiki

Safety considerations

The Setup operates with high currents and voltages and is therefore dangerous for the operator and other instruments. Dangers include but are not limited to:

  • Electrical discharged
  • Heat / Cold burns from the Peltiers
  • Water damage in case of leakage

Whenever anything on the setup is changed, make sure to check the proper operation of the setup.


Before any usage of the setup it should be checked that it is working as inteded (off the microscope).

Check before every usage:

  • Wire connections
  • Thermistor placement
  • Tightness of all tube conenctions and Peltier - Backplate connection

Do never power the Arduino through the USB connection when the power switch is turned ON, as this can damage the Arduino and driver boards.

Motor calibration

Since the motor can be moved when the setup is powered off it can happen that the motorposition on the Arduino is not accurate. If the motor drives to the end of the travel, it is possible that the motor damages itself. Therefore it is important to check the motor calibration every time the motor is used. To do this carefully drive the motor to the zero position and check, wether it is the real zero position as indicated by the alignement on the motor and the backplate. If this is not the case, move the motor to the real middle position and re-calibrate the zero position by pressing Setting/Calibrate. The motor can for this also be moved by disabling Settings/motor hold and turning the barrel on the opposite end of the motor.

Condensation prevention

Humidity can be a large problem for the experiment, either because it makes imaging of the sample impossible or it can lead to frost on the sample movement frame and prevent sample translation. Therefore it is important to check and close all places where humidity could enter the setup. It is good practice to put a glass slide with dessiccator beads on it onto the copper block during the experiment. Furthermore any condensation should be removed before the Setup is closed. After the experiment it should be studied where frost / condensation appeared in the setup and the enclosure should be optimised accordingly for the next experiment