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Correct protein sequence from GenBank AY593970, confirmed by ESTs for both genes on Chr2 duplication:

>Protein gene: cpnB
MTTPISLSKPRVELRFKCSHLKNLDICSKSDPLIMVFDKRGEHGESIFVGQTEKINNNLN
PEFKKSVIIDYHFENIQNLSFIVVDIDKEIKRVGDLEGNDIIGQYTTTLGNIISKPNKKV
ISEIKHKGKETGVIEITAEEIRETGHNFLFKINGTKLDKKDLFTSDPYFKIYKTSSSGNV
LVYQSVVIKNTLNPNYEPVMMKLEELNNGDMFRELVFEFWDHDSVGEHDFIGLFTTNADT
VLKGITREFPLINAKKAAKKSGSYKNSGVITFTDCRLVGQPTFIDYLSGGCEINLMVAID
CTASNGSPSTSTSLHYHTPSHPSQYAKSIFSVGSVLAPYDSDGNIEVLGFGGIHRGSTSH
CFQFGSKKEVRGVEGVLLTYGEVIPSMSLSYPTNFKDIISYAAKKSLDGVTSKSQKYTIL
LILTDGEISDMNETVHEIVKASKQSPLSIVIIGIGEATFDNMNRLDGDDGNSLTDSSGQT
ATRDIVQFVPFNNFSANPEALATETLREIPQQLLGFMNQNNYLPMSHRLK

pfey May 2020

Protein alignment with correct protein from GenBank AY593970 on top and protein derived from genomic at bottom.

AY593970: 1   MTTPISLSKPRVELRFKCSHLKNLDICS-KSDPLIMVFDKRGEHGESIFVGQTEKINNNL 59
              MTTPISLSKPRVELRFKCSHLKNL     KSDPLIMVFDKRGEHGESIFVGQTEKINNNL 
Genomic:  1   MTTPISLSKPRVELRFKCSHLKNLGKYQYKSDPLIMVFDKRGEHGESIFVGQTEKINNNL 60

AY593970: 60  NPEFKKSVIIDYHFENIQNLSFIVVDIDKEIKRVGDLEGNDIIGQYTTTLGNIISKPNKK 119
              NPEFKKSVIIDYHFENIQNLSFIVVDIDKEIKRVGDLEGNDIIGQYTTTLGNIISKPNKK 
Genomic:  61  NPEFKKSVIIDYHFENIQNLSFIVVDIDKEIKRVGDLEGNDIIGQYTTTLGNIISKPNKK 120

cDNA alignment of GenBank AY593970 (also supported by ESTs) on top and cDNA from genomic sequence with insertion, mismatches and deletion at bottom.

AY593970: 1    atgacaacaccaattagtttaagtaaacccagagtagaattgagatttaaatgttcacat 60
               |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| 
Genomic:  1    atgacaacaccaattagtttaagtaaacccagagtagaattgagatttaaatgttcacat 60

AY593970: 61   ttaaagaatttgg----atatttgtagtaaatcagatccattgattatggtttttgacaa 116
               |||||||||||||    ||||   || ||||||||||||||||||||||||||||||||| 
Genomic:  61   ttaaagaatttgggtaaatatcaata-taaatcagatccattgattatggtttttgacaa 119

AY593970: 117  aagaggtgaacatggtgaatcaatatttgtaggacaaactgaaaaaattaacaataattt 176
               |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| 
Genomic:  120  aagaggtgaacatggtgaatcaatatttgtaggacaaactgaaaaaattaacaataattt 179

pfey July 2022

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