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DDB_G0280079 codes for a protein with significant homologies to the transcriptional ADAptor subunit ADA2 of budding yeast. It is the first and by far the best BLAST hit for yeast ADA2 in Dicty, as well as for the recognized human ADA2 ortholog and suspected plant orthologs. The homology covers virtually the entire length of the yeast, human and plant proteins. The Dd protein has, in addition, an N-terminus of some 400 residues which has no correspondence in the sequences from yeast or multicellular organisms; this N-terminus appears unique to Dd, as it is not conserved in either Dp or Pp.

Yeast ADA2 participates in several complexes together with the histone acetyl transferase GCN5, which also has an ortholog in Dicty. The yeast gene is not essential for viability, but mutants show slow growth. ADA2 has been shown to increase the HAT activity of GCN5 (for references see (https://www.yeastgenome.org/locus/S000002856).

The yeast gene shows a weak but fairly convincing cell cycle regulation, with the expression maximum in the S-phase. DDB_GO280079 appears likely to be cell-cycle regulated in Dicty, as it is threefold overexpressed in a strain in which the Dicty Rb ortholog rblA has been disrupted. Most genes overexpressed in this strain have roles in cell cycle progression, and most genes with known roles in cell cycle progression are overexpressed in this strain.

In addition, DDB_G0280079 shows a developmental trajectory similar to those of most genes involved in cell cycle progression (see DictyExpress). Finally, the promoter contains an element (GCGCCCAAA) which is highly enriched in the promoters of Dictyostelium cell cycle genes.

It seems reasonable to suppose that DDB_G0280079 is part of the Dictyostelium cell-cycle transcriptional hierarchy. Its target genes in Dicty are at present unknown.

Harry MacWilliams, June 2010

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