DDB_G0293164 - dictyBase/community-annotations GitHub Wiki

DDB_G0293164 protein sequence as derived from EST SSA713 (DDB0297897) with the missing Y 3 residue and all changed residues shown below.

MIYKKWIILVFLINCFLFVQSLDKKGIYKDNRDSGKSLVLDGKSDYLSSSIIIGFDTFNV
IIDTTLNYLIVAERKCDGCTQYPPYYRESQVFENIQCDSPACHSIGNTCDKPNKYDPPTC
GYETKLQQYGANIKTILYRDIISIDDFKNIPVIISAIYEQRNGLSLNSAILGVGPSCSTC
PPSPLQSLLYTLKKPYIFGISLDKNYFGGISIGKIDPLLYTGVINYTTMSKRDGVYSVSP
IFIGSHWNDQIYNSTRNDIKRFIISSSSSLSYIPTSPYKQLKQFIKSAGCKSGDLDFCSK
IDSLFNSCINSSQISISRFPDIDLYFEKGFILTIPPKIYFHSISRNNQVFICMGILESTD
TNAILGINLMRELYTVFDNEKTLIGFSRK

pfey May 2011

Alignment of EST SSA713 sequence on top and genomic sequence at bottom; the first ATG in the top line is the initiation codon; note the stop codon (TAA instead of TAT) in the genomic sequence (bottom) in the 3rd codon after the initiation ATG.

correct  12   AAAATATAAAAAAAATTAAAAAAAAAAAAATGATTTATAAAAAGTGGATAATTTTAGTAT  71
              |||||||||||||| |||||||||||||||||||||| |||||| ||  | |||  | ||
genomic  1    AAAATATAAAAAAATTTAAAAAAAAAAAAATGATTTAAAAAAAGGGGGAATTTTGGGGAT  60

correct  72   TTTTAATAAATTGTTTTTTATTTGTTCAAAGTTTGGATAAAAAAGGGATTT  122
              |||||| |||||||||||| ||||||||||||||||| ||||||||| |||
genomic  61   TTTTAAAAAATTGTTTTTTTTTTGTTCAAAGTTTGGAAAAAAAAGGGGTTT  111

Alignment of the N-terminal protein sequence with the correct sequence derived from EST SSA713 on top and protein from genomic DNA at the bottom; note the asterisk in the 3rd position and the missing Y residue in the genomic derived sequence, as there is a stop codon in the genomic sequence and an artificial gap in the curated model keeps the sequence in frame.

correct  1    MIYKKWIILVFLINCFLFVQSLDKKGIYKDNRDSGKSLVLDGKSDYLSSSIIIGFDTFNV  60
              MI KK     FL NCF FVQSL+KKGIYKDNRDSGKSLVLDGKSDYLSSSIIIGFDTFNV
genomic  1    MI*KKGEFWGFLKNCFFFVQSLEKKGIYKDNRDSGKSLVLDGKSDYLSSSIIIGFDTFNV  59

correct  61   IIDTTLNYLIVAERKCDGCTQYPPYYRESQVFENIQCDSPACHSIGNTCDKPNKYDPPTC  120
              IIDTTLNYLIVAERKCDGCTQYPPYYRESQVFENIQCDSPACHSIGNTCDKPNKYDPPTC
genomic  60   IIDTTLNYLIVAERKCDGCTQYPPYYRESQVFENIQCDSPACHSIGNTCDKPNKYDPPTC  119

pfey April 2022

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