DDB_G0292124 - dictyBase/community-annotations GitHub Wiki

DDB_G0292124 protein sequence as derived from sequence similarity including the Leucine 381, shown below; an artifial gap had to be introduced because of a frame shift in the genomic sequence.

MVRINKYKHKHDGTDEFFEITDYTQASSWENLIAKLEEVISSSCENRKSSFDHSFQFNNT
NFQLVYISPINTLSLSKQEISKISKLKYNGFMNAKNDFQYKFINNDNKENNNNNNNSTSN
NNNNNNIYQVENFLNWFGIQEYFLMLAMRDNGPNVTDLSTIMSAFSIALHNCNCYLPAFV
GFDEKRYENFLGYMYAPISSRSIDNEDHFFVKFATESIVLSTLTSNQNISESFEKQLRIF
YSKISVDLDDRNWDQFLALSTTSTKYTYIKGNFDDLQWKQSSNNSNLKNNNKNNNNKNNR
QINNNNNSSIGDEDENDDEENQRIKNSNNGGGGGGGGGGGGGDGSGGNSDGDIFKWGYPM
DPINELHWCVMCPPIKSTHILENDFQSIQTPKSWFIKVVFRKPHNSLFQLSIGVINVFTS
YVEAIKSSNQSIQQILSDYNLYKSNSSSPITTTTTTTTNYQQQHYKQNLDQQQHQQHQQQ
QQQQQYEDTSILKTMGTFTKSLASSLSSPLIPTPKELDMLLHDLFFETKDPSDIYHFNNI
PEEELNQSPLPNKNSRIKRSPIDSLFFSFSIMCLNIQSLVGILLFWNEFVEEIKWHWEHQ
ILIPRTFSSSHISVNNCLIFQKLQMINYCIKRKQEYDHIFQSNENLENNNNQKQQQQSQN
NNNNNQDGWDDDDDDIIPVIDDEKEQDGWDDFEDPLEGLDDDDNNNNNNNNKKEEEEGEE
IELKYSGGGNIKKLNGQYLLYHDKEIIIPETQEFGPMTDDMVQDQLNEMSDINISQEKRI
EMQTVSLLSDMQSFKYFNPGSVFEDFIRWHSNTDWITKPSEIESIEKRLLQEKQNKQKEN
QVNNNNNNNNNNNNNNNNNSDEDIVVVDNNNNDDNDDDDDDDDDDDDDDDDLIKPIIETT
LNDELIQIIDKFDESKGIERKSGFGRDGCLSLRMAGNHTIWRKTWIKSKPLPISKQKQLF
DHTKQAIKSLNYLESVSPSDLVHQIMSVILTSITTIFSNEKAKSNFSSEYCLGIDFQPLK
QLVEKFFDTVNSIWPSQIPHYESIKTKDFDPIFKNLQDIENSYSKLISLKTKFSKLDRVI
FNLYRDGYSDILDKEFESISYLFFNELDDYEYEMENGDGIPSSIQPNIKEYITRSYSPRP
YKSSQMMPHKMYTCISPYECRISTSISEEDL

pfey May 2011

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Protein alignment of corrected protein on top and curated model from genomic sequence at bottom.

Correct 362    DPINELHWCVMCPPIKSTHILENDFQSIQTPKSWFIKVVFRKPHNSLFQLSIGVINVFTS  422
               DPINELHWCVMCPPIKSTHI ENDFQSIQTPKSWFIKVVFRKPHNSLFQLSIGVINVFTS
Genome  362    DPINELHWCVMCPPIKSTHI-ENDFQSIQTPKSWFIKVVFRKPHNSLFQLSIGVINVFTS  421

Alignment of the same group 4 Dictiostelid D. purpureum sequence at bottom, D. discoideum curated model sequence on top.

Genome: 353 IFKWGYPMDPINELHWCVMCPPIKSTHI-ENDFQSIQTPKSWFIKVVFRKPHNSLFQLSI 411
            IF+WGYPMDPINELH+CV+ PPIKSTHI ENDFQS QTPKSWF+KV+FRKPHNSLFQLSI 
D. pur: 241 IFRWGYPMDPINELHYCVISPPIKSTHILENDFQSSQTPKSWFVKVLFRKPHNSLFQLSI 300

Alignment of group 2 Heterostelium album (former P. pallidum) at bottom, D. discoideum curated model sequence on top.

Genome:   353 IFKWGYPMDPINELHWCVMCPPIKSTHI-ENDFQSIQTPKSWFIKVVFRKPHNSLFQLSI 411
          +F+WGYPMDPI +L  CVM PP+ S H+ EN  ++IQT KSWFIK VFRK H SLFQLS+ 
H. album: 303 LFRWGYPMDPIEQLQLCVMYPPLASIHVLENQLENIQT-KSWFIKAVFRKSHGSLFQLSL 361

In the alignments of two other Dictyostelids, one phylogenetic near and the other more distant, both have the conserved L residue in place where the genomic D. discoideum sequence seems to have a 1 nt deletion. The codon had to be deleted by adding an artifial 2 nt gap to stay in frame.

pfey April 2022

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