DDB_G0274705 - dictyBase/community-annotations GitHub Wiki

The DDB_G0274705 protein sequence including the valine at position 162 that is missing in the curated gene model:

MIEINNKIDYIFYTNNTPNTYKIQLILLELQKEYGITFESRFINVFKKENYSDDYIKINP
NKKVPAIVDQTGEKPFIVFESVSILIYLAQKFNTFLPDFKTNPLENSEVITWTVWQAANL
GPAFGQYFHFSFFSPTVQEYSLHRFNNEAQRILRLLDDRLSVSTYIGGNEFSIADIASAG
WLLYLNSAPLYNATKERFPNIFKWLDLINQRESIKQVNQSISDGFKNFNPSLLRALFTND
PELINAKAPIGIENVILKYD

pfey December 2008

Protein alignment of correct sequence derived from ESTs (DDB0164011, DDB0023106) on top with dictybase curated model derived from the genomic sequence, below; an artificial gap has been introduced to keep the gene and thus protein in frame.

Correct: 121 GPAFGQYFHFSFFSPTVQEYSLHRFNNEAQRILRLLDDRLSVSTYIGGNEFSIADIASAG 180
             GPAFGQYFHFSFFSPTVQEYSLHRFNNEAQRILRLLDDRLS STYIGGNEFSIADIASAG 
Genomic  121 GPAFGQYFHFSFFSPTVQEYSLHRFNNEAQRILRLLDDRLS-STYIGGNEFSIADIASAG 179

Nucleotide alignment with genomic DNA on top and EST DDB0023106 sequence at bottom; note the missing t nucleotide in the genomic sequence.

Genome: 1620 tcaagaatattctttacatagatttaataatgaagcacaacgtattcttagattattaga 1679
             |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| 
EST:    68   tcaagaatattctttacatagatttaataatgaagcacaacgtattcttagattattaga 127

Genome: 1680 cgatagattatcag-atcaacatatattggtggaaatgaattttctattgcagatattgc 1738
             |||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||| 
EST:    128  cgatagattatcagtatcaacatatattggtggaaatgaattttctattgcagatattgc 187

Genome: 1739 aagtgcaggttggttattatacttaaactctgcaccattatataatgcaactaaagaacg 1798
             |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| 
EST:    188  aagtgcaggttggttattatacttaaactctgcaccattatataatgcaactaaagaacg 247

pfey March 2022

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