Other - coyotespike/LH GitHub Wiki

When is LH sent to the skin? Is skin affected by the LH surge?

1. How to detect LH chemically?

Antibodies, reacted with gold conjugate.

1. How to detect in the skin? Is it possible?

Test strips on Rhiannon sweat. Get antibodies, conjugate reactant, try more sensitive doses. Make list of other hormones and potential methods of detection.

1. How to turn into product?

External epidermis (bracelet, just a patch that changes color, watch with phases) Urine (device in toilet?) Blood (implant?)

What makes the chemistry degrade when exposed to the air? How to make more stable? Can the test and control be made smaller?

Planned our test in more detail.

About a week before LH surge was anticipated, female subject began daily urine and perspiration tests. After the control urine LH test returned positive, a modified sweat test, with a more optimal technology, was also tested.

Then we test with 5-10 other females. At that point, we have a scientific discovery, and technolgoy proof of concept.

If it does not work, then we need to consider entirely different avenues.

If it does, we should focus on developing the product.

We should also think of non-chromatographical tests.

If it doesn't work, it can be due to underlying biological reasons, or to technological reasons. Maybe our tests didn't work. Maybe the LH surge doesn't affect the skin in a detectable way.

Other hormones which may be used to detect, and how to detect those hormones biologically, and technologies to detect them.

LH: where in the body to detect, and other tests to use. Blood monitor? Other skin device?

Bookface Tim Ward Study authors?

Research antigens and antibodies Research other hormones and tests for them Research bodily presence of sex hormones Research nanotech tests (how do blood monitors work?). Consider whether to compete with eightsleep.

Figure out how to manufacture these patches.

How to make our own? How to source our own?

What's the best pathway of sweat to the patch? From underneath, or to the top? Will the antibodies react in the air? Are there obvious stability problems?

Where to get it industrially manufactured? How can we make our own?

Maybe the patch is coated with something that dissolves in sweat.

Why shouldn't the test be left out? Because it is physically harmed, and now not enough can react to trigger the test? Or because it chemically reacts with biologically homologous substances?

In dogs the test is performed on the vaginal fluid, raising the question: can LH be detected in the cervical fluid? Most women do not know how and do not want to know how to assess the quality of their cervical fluid, much less to record it. We need smart underwear to assess whether the cervical fluid is fertile-quality and whether it contains LH.

Is it merely a proxy to measure estrogen? Or can estrogen level serve as a proxy for cervical fluid?

Spinnbarkeit and fern test (estrogen, sodium chloride).

http://blog.kindara.com/blog/the-many-faces-of-cervical-fluid

May be significantly less annoying to test LH inside the vagina than from a cup of urine.

Second test invented: test for LH on cervical fluid.

Vaginal fluid. Sweat. Saliva.

Will nanotech enable us to create a hormone sensor, which is permanent/stable/reusable?

Why gold conjugate? Any other metal conjugate?

Hypothesis: using this as a patch will not work, but will lead to false positives instead. However, one should be able to dab it at the sweat, and in a patch form this will work even better. An LH sweat dab.

A patch would eliminate the need to use a cup for urine collection, because it eliminates the possibility of splashing - that is, the possibility that bodily fluids could reach the test area without having first passed through the gold conjugate layer.

• Smart fabric for pants?
• Implanted sensor?
• Stick-on paper patch?
• Sensor for vagina?
• Sensor for mouth, for sweat? Combined with thermometer?
• Optic sensor, like Echo Labs? Need to research possibility.