4 Using DIMet Galaxy - cbib/DIMet GitHub Wiki

DIMet is available in Galaxy Europe, visit us in https://usegalaxy.eu/

New: DIMet is now also available in Workflow4Metabolomics (W4M) at https://workflow4metabolomics.usegalaxy.fr/. All the instructions are the same for both Europe and W4M instances of Galaxy.

DIMet Galaxy is composed of several modules, each one has its own user guide. You can find each module easily by typing 'dimet' in the search bar:

Modules for data processing:

• dimet_pca_analysis computes the PCA and outputs tables with principal components and explained variances Link

• dimet_differential_analysis runs pair-wise (2 groups) differential analysis and computes the corresponding statistics Link

• dimet_multi_group_comparison same as differential analysis before, but for > 2 groups Link

• dimet_timecourse_analysis runs differential analysis for time-course experiments in pairwise fashion for consecutive time points Link

• dimet_bivariate_analysis runs the correlation test to compare MDV profiles, or the metabolite time course profiles Link

Modules for visualization:

• dimet_pca_plot generates classical PCA plots Link
• dimet_abundance_plot plots with bars of total metabolite abundancest, by metabolite Link
• dimet_enrichment_plot generates lineplots of mean enrichment, by metabolite Link
• dimet_isotopologues_plot generates stacked bars of isotopologue proportions, by metabolite Link

Module for integration of (tracer) metabolomics and transcriptomics

• dimet_metabologram pathway-based integration between labeled targeted metabolomic and trascriptomic data, resulting in metabologram plots Link

Each module has its own documentation in the DIMet Galaxy interface.