A3 Journal - bcb420-2024/Dien_Nguyen GitHub Wiki

Thresholded overrepresentation analysis

  • I decided to use GSEA because it's one of the best tools and easy to use. There's also a lot of documentation on GSEA.
  • Since there was no GSEA for R, I first opted for an R package called GSEA_R. However, the Rstudio server crashes every time I run the gsea function.
  • fgsea is another option I tried. However, the results are not formatted to work with Cytoscape, so it produced an error. Some extra columns have to be added in order for Cytoscape to properly parse it.
  • Finally decided to use the desktop GSEA tool in the end since it's straight-forward, and there's less space for error.
  • Used this geneset
  • Results:
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