24‐Dehydrolathosterol: Trusted Cholesterol Impurity Standard for Advanced Pharmaceutical Research - aquigenbio/blog GitHub Wiki

In the realm of pharmaceutical R&D, where precision and compliance are non-negotiable, the quality of reference standards plays a pivotal role. Among the most specialized of these are cholesterol impurity standards — used extensively in method development, validation, and regulatory filing for formulations and active pharmaceutical ingredients. One such high-value standard is 24-Dehydrolathosterol, a compound that offers critical analytical support in sterol-related drug profiling.

What is 24-Dehydrolathosterol? 24-Dehydrolathosterol (CAS Number: 17608–47–8) is a sterol intermediate in cholesterol biosynthesis. It features a distinct molecular structure, with the formula C₂₇H₄₄O and a molecular weight of 384.64 g/mol. Its unique configuration makes it particularly relevant for research and analytical applications that require accurate quantification and separation of cholesterol-related impurities.

At Aquigen Bio, this compound is available under the catalog number AQ-C004235 and is typically in stock for immediate delivery, with dispatch available within 24 hours. The product is handled under strict quality conditions and includes comprehensive documentation such as a Certificate of Analysis (CoA), Mass Spectra, IR spectra, and complete NMR data.

Importance in Analytical Method Development and Validation 24-Dehydrolathosterol is widely used in the development and validation of chromatographic methods, such as HPLC or GC-MS, where it acts as a critical impurity reference. It supports the identification, separation, and quantification of related substances in cholesterol-based APIs or formulations. This is particularly important in the context of:

Abbreviated New Drug Applications (ANDAs) Stability studies Pharmacokinetic modeling Residual impurity profiling Regulatory bodies like the FDA and EMA require detailed impurity profiling as part of the drug approval process. In these cases, validated methods incorporating high-purity standards like 24-Dehydrolathosterol provide the confidence and reliability necessary for both internal QA processes and external audits.

Traceability and Compliance with Pharmacopeial Standards Aquigen Bio ensures that 24-Dehydrolathosterol is aligned with global regulatory expectations. Whenever possible, the reference material includes traceability to pharmacopeial standards such as USP or EP, making it highly compatible with documentation requirements for global submissions. All standards are manufactured under controlled conditions to ensure reproducibility, homogeneity, and long-term stability.

Strictly for Analytical Use It is critical to note that 24-Dehydrolathosterol, like all impurity standards offered by Aquigen Bio, is intended strictly for laboratory and analytical use. It is not for human consumption, formulation, or therapeutic use. This standardization ensures that laboratories using these materials maintain a high degree of safety and regulatory alignment during their development programs.

Category Context: Cholesterol Impurity Standards 24-Dehydrolathosterol is part of Aquigen Bio’s broad Cholesterol Impurity Standards category, which includes sterol derivatives, biosynthetic intermediates, and oxidized sterol analogs. This category supports comprehensive sterol profiling across pharmaceutical, nutraceutical, and biochemical research applications.

Whether you’re working on sterol metabolism research, formulating lipid-based drug delivery systems, or monitoring degradation pathways in storage conditions, the cholesterol impurity standards collection from Aquigen Bio offers high-purity materials to support your needs.

Related Products That Complement 24-Dehydrolathosterol To support complete profiling and robust method validation, Aquigen Bio recommends complementary cholesterol-based reference standards. Two particularly noteworthy products include 27-Hydroxy Cholesterol-d₆ and 24-Ketocholesterol.

1. 27-Hydroxy Cholesterol-d₆ Deuterated standards have become invaluable tools in modern bioanalytical chemistry, especially when working with LC-MS/MS systems. 27-Hydroxy Cholesterol-d₆ (CAS Number: 1246302–95–3) is a stable isotopically labeled analog of 27-hydroxy cholesterol. Its chemical structure incorporates six deuterium atoms, providing a reliable internal standard for accurate quantification of cholesterol metabolites.

With a molecular formula of C₂₇H₄₀D₆O₂, this compound is especially useful for:

Calibration curve preparation Matrix spike recovery studies Quantitative pharmacokinetic analysis Complex biological sample analysis (e.g., plasma, tissue) Its isotope labeling minimizes variability and enhances reproducibility in mass spectrometric detection, making it an essential tool for both research and regulatory validation. The product is shipped with complete spectral data, including deuterium NMR, and is aligned with global QA expectations.

More info: 27-Hydroxy Cholesterol-d₆ product page

2. 24-Ketocholesterol Another related impurity standard is 24-Ketocholesterol (CAS Number: 17752–16–8). This sterol derivative is part of the oxidized cholesterol family and is a critical impurity reference for oxidative stress studies and long-term stability evaluations in lipid-containing formulations.

With a molecular weight of 400.6 g/mol, this compound is routinely used for:

Oxidative degradation analysis in formulations Research into cholesterol oxidation products (COPs) Stress testing under ICH conditions Drug interaction and metabolism modeling Like other Aquigen Bio products, 24-Ketocholesterol is supplied with full CoA and analytical data, offering confidence in its performance and traceability.

Explore this product here: 24-Ketocholesterol product page

Why Choose Aquigen Bio for Impurity Standards? Aquigen Bio stands out as a dedicated supplier of pharmaceutical impurity standards, offering a wide range of high-purity compounds backed by complete analytical documentation and rapid fulfillment timelines. Their commitment to scientific integrity, regulatory compliance, and customer support ensures that your R&D timelines remain on track without compromising analytical quality.

With a product portfolio that spans from cholesterol-related impurities to advanced isotope-labeled compounds, Aquigen Bio offers everything modern pharmaceutical labs need to fulfill analytical, validation, and regulatory obligations.

Key Benefits: Ready stock with 24-hour dispatch for most products USP/EP traceability wherever applicable Complete documentation (CoA, MS, NMR, IR) Strictly for analytical purposes (non-clinical, non-human use) Competitive global shipping Conclusion 24-Dehydrolathosterol represents more than just another sterol impurity — it is a vital analytical tool that empowers pharmaceutical researchers to build accurate, reproducible, and regulatory-compliant data around cholesterol-based molecules. When paired with related standards like 27-Hydroxy Cholesterol-d₆ and 24-Ketocholesterol, it provides a comprehensive framework for sterol impurity profiling and method validation.

By choosing Aquigen Bio’s impurity standards, researchers can work with confidence, knowing that every compound has been tested and documented to meet the highest levels of quality and precision. If you are involved in cholesterol pathway research, lipid-based formulation development, or analytical quality control, 24-Dehydrolathosterol and its related reference standards deserve a place in your lab’s toolkit.