Welcome to the LEMOLISH wiki (under construction)!

LEMOLISH is an open-source device, composed of easily accessible LEGO® and LEGO-MINDSTORMS® parts, simple optics and electronics, labware and software code. It is designed to enable 3D imaging of large (0.5-5cm) and optically cleared organs and organisms with lightsheet illumination and orthogonal camera-based detection. LEMOLISH is a LEGO®-based version of a SPIM (Single-Plane Illumination Microscope) that enables double sides detection with a horizontal layout.

LEMOLISH aims to make cleared organs imaging accessible to any laboratory/institution for scientific imaging and advanced teaching, and offers an price tag starting below 2000 Euros.

DISCLAIMER: LEMOLISH is not a toy!! Its scientific performances are reached only through the use of moderate-power laser diodes which must be manipulated carefully.

It consists of five parts

How it works

Find here the essentials to understand LEMOLISH, including the background info about Lightsheet Microscopy and where LEMOLISH stands in terms of layout and applications, the optical architecture, the expected performance, and its limitations too.

How to build it

The instructions to construct your own LEMOLISH: LEGO® Architecture (Including two versions, basic and complete), step-by-step manuals, explanation of the gear mechanics driving the parts, tips and tricks

Optics: tutorial on how to build and align LEMOLISH optics.

Electronics: two ways to synchronize the EV3 brick to the cameras and lasers.

How to use it

A guide through the use of the LEGO® MINDSTORMS® EV3 intelligent brick that drives image acquisition

How to process multiview Images

Two simple and open source software tools to recombine two-sided detection and sample-rotated (0º/180º) image stacks for improved lightsheet imaging on LEMOLISH.

How to buy parts

a purchase guide through the optics, electronics, labware and LEGO®-based components.