Week 3 Jan 29 Feb 4 - WangXueqing007/Master_Thesis GitHub Wiki

Jan 29:

  • I want to develop a solid pipeline for defining grids and turn the channel into an appropriate coordinate--is that doable?
  • I don't feel like that turning the coordinate is a good idea...It is not easy to visualize in 3D space, and the angle is not accurate. I would rather adjust the grid instead...

Jan 30:

  • Today: Docked lindane to the pore area of one isoform in 4npq. In 4 folders.
  • Repeat three times in AutoDock manually.
  • Have to figure out how t do "high-throughput" in bash.
  • Learn more about coordinate adjusting in VMD.
  • Will do: docking of PFL to 5MUO.
  • More notes on notebook.

Jan 31:

  • PFL-5MUO result: doesn't bind to the position in co-crystallization structure.

Dec 2:

  • GLIC Meeting:
    • Sarah: Docking is perhaps better for checking the difference among different compounds than finding different binding positions of one compound?
    • The contradictory results: could be that PFL and lindane are not very specific bound in GLIC? PFL rather binds in the subunits?
    • To do: build up the statistics; look into fipronil?
    • Fipronil: on one hand more specific binding; on the other hand limited (?)
    • Clustering?
  • What I did today:
    • Two meetings.
    • Tried VMD VolMap Tool--> choose "pdb" as input file type.
    • Read a bit into the third tutorial.
    • Figured out the clustering.sh script, tried to download GROMACS...
  • To-do list!
    • Linux basic--shell script...
    • Figuring out a good way to present the docking results: Ideally, by next Friday, docking results of PFL, lindane, fipronil, to two structures (perhaps 20x20?).
    • Install GROMACS, install GROMACS, install GROMACS !!!
    • Intro writing?
    • By the end of today, finish the 30-min Linux guide!