raw data analysis - WXlab-NJMU/scrna-recom GitHub Wiki
Tools: cellranger
Please CellRanger first, and add the installed folder to PATH
.
scrna-rawdata <indir> <outdir> <project> <genome>
Raw Data Processing Using CellRanger
positional arguments:
indir input directory
outdir output directory
project project name
genome cellranger genome path
options:
-h, --help show this help message and exit
--dformat DFORMAT data format: BCL or Fastq
--index INDEX barcode csv, header is Lane,Sample,Index
--fq_path FQ_PATH fastq csv, header is Lane,Sample,FastqDir
--multi MULTI whether to run multi, please set True if using cell multiplex
--aggr AGGR whether to run aggr(normalize,reduction,cluster), please set True if using Loupe Viewer