communication - WXlab-NJMU/scrna-recom GitHub Wiki
Tools: CellChat
Rscript cell-communication.R <input> <outdir> <project> [options]
scRNA-seq Cell Communication Analysis using CellChat
positional arguments:
infile input SeuratObject rds, Idents should be set to celltype
outdir output result folder
project project name
flags:
-h, --help show this help message and exit
optional arguments:
-x, --opts RDS file containing argument values
-m, --method current support is CellChat [default: CellChat]
-s, --species human or mouse [default: human]
-c, --cores cores to do parallel [default: 20]
# SingleR
Rscript cell-communication.R examples/two-samples.rds ~/tests/communication test
communication
├── test.interaction.cellchat.init.rds
├── test.interaction.cellchat.infer.rds
├── test.interaction.cellchat.inferred_network_ligands.tsv
├── test.interaction.cellchat.inferred_network_pathway.tsv
└── test.interaction.cellchat.output.pdf