Output - UPHL-BioNGS/Cecret GitHub Wiki

Output or results

Cecret produces a lot of files. Most of these files are in the format of {params.outdir}/{process}/{resultant files from that process}.

Tree of summary files

Final File Tree after running cecret.nf
cecret/
├── aligned
│   ├── ERR5181310.sorted.bam
│   ├── ERR5181310.sorted.bam.bai
│   ├── ERR5181360.sorted.bam
│   ├── ERR5181360.sorted.bam.bai
│   ├── ERR5405022.sorted.bam
│   ├── ERR5405022.sorted.bam.bai
│   ├── ERR5556343.sorted.bam
│   ├── ERR5556343.sorted.bam.bai
│   ├── ERR5691945.sorted.bam
│   ├── ERR5691945.sorted.bam.bai
│   ├── ERR5743043.sorted.bam
│   ├── ERR5743043.sorted.bam.bai
│   ├── ERR5743893.sorted.bam
│   ├── ERR5743893.sorted.bam.bai
│   ├── ERR5749609.sorted.bam
│   ├── ERR5749609.sorted.bam.bai
│   ├── ERR5771988.sorted.bam
│   ├── ERR5771988.sorted.bam.bai
│   ├── SRR13422799.sorted.bam
│   ├── SRR13422799.sorted.bam.bai
│   ├── SRR13422802.sorted.bam
│   ├── SRR13422802.sorted.bam.bai
│   ├── SRR13500958.sorted.bam
│   ├── SRR13500958.sorted.bam.bai
│   ├── SRR13511054.sorted.bam
│   ├── SRR13511054.sorted.bam.bai
│   ├── SRR13603869.sorted.bam
│   ├── SRR13603869.sorted.bam.bai
│   ├── SRR13606410.sorted.bam
│   ├── SRR13606410.sorted.bam.bai
│   ├── SRR14241199.sorted.bam
│   └── SRR14241199.sorted.bam.bai
├── bcftools_variants
│   ├── ERR5181310.vcf
│   ├── ERR5181360.vcf
│   ├── ERR5405022.vcf
│   ├── ERR5556343.vcf
│   ├── ERR5691945.vcf
│   ├── ERR5743043.vcf
│   ├── ERR5743893.vcf
│   ├── ERR5749609.vcf
│   ├── ERR5771988.vcf
│   ├── SRR13422799.vcf
│   ├── SRR13422802.vcf
│   ├── SRR13500958.vcf
│   ├── SRR13511054.vcf
│   ├── SRR13603869.vcf
│   ├── SRR13606410.vcf
│   └── SRR14241199.vcf
├── cecret_results.csv
├── cecret_results.txt
├── consensus
│   ├── ERR5181310.consensus.fa
│   ├── ERR5181360.consensus.fa
│   ├── ERR5405022.consensus.fa
│   ├── ERR5556343.consensus.fa
│   ├── ERR5691945.consensus.fa
│   ├── ERR5743043.consensus.fa
│   ├── ERR5743893.consensus.fa
│   ├── ERR5749609.consensus.fa
│   ├── ERR5771988.consensus.fa
│   ├── SRR13422799.consensus.fa
│   ├── SRR13422802.consensus.fa
│   ├── SRR13500958.consensus.fa
│   ├── SRR13511054.consensus.fa
│   ├── SRR13603869.consensus.fa
│   ├── SRR13606410.consensus.fa
│   └── SRR14241199.consensus.fa
├── dataset
│   ├── CHANGELOG.md
│   ├── genome_annotation.gff3
│   ├── pathogen.json
│   ├── README.md
│   ├── reference.fasta
│   ├── sequences.fasta
│   └── tree.json
├── fastqc
│   ├── ERR5181310_1_fastqc.html
│   ├── ERR5181310_1_fastqc.zip
│   ├── ERR5181310_2_fastqc.html
│   ├── ERR5181310_2_fastqc.zip
│   ├── ERR5181310_fastq_name.csv
│   ├── ERR5181360_1_fastqc.html
│   ├── ERR5181360_1_fastqc.zip
│   ├── ERR5181360_2_fastqc.html
│   ├── ERR5181360_2_fastqc.zip
│   ├── ERR5181360_fastq_name.csv
│   ├── ERR5405022_1_fastqc.html
│   ├── ERR5405022_1_fastqc.zip
│   ├── ERR5405022_2_fastqc.html
│   ├── ERR5405022_2_fastqc.zip
│   ├── ERR5405022_fastq_name.csv
│   ├── ERR5556343_1_fastqc.html
│   ├── ERR5556343_1_fastqc.zip
│   ├── ERR5556343_2_fastqc.html
│   ├── ERR5556343_2_fastqc.zip
│   ├── ERR5556343_fastq_name.csv
│   ├── ERR5691945_1_fastqc.html
│   ├── ERR5691945_1_fastqc.zip
│   ├── ERR5691945_2_fastqc.html
│   ├── ERR5691945_2_fastqc.zip
│   ├── ERR5691945_fastq_name.csv
│   ├── ERR5743043_1_fastqc.html
│   ├── ERR5743043_1_fastqc.zip
│   ├── ERR5743043_2_fastqc.html
│   ├── ERR5743043_2_fastqc.zip
│   ├── ERR5743043_fastq_name.csv
│   ├── ERR5743893_1_fastqc.html
│   ├── ERR5743893_1_fastqc.zip
│   ├── ERR5743893_2_fastqc.html
│   ├── ERR5743893_2_fastqc.zip
│   ├── ERR5743893_fastq_name.csv
│   ├── ERR5749609_1_fastqc.html
│   ├── ERR5749609_1_fastqc.zip
│   ├── ERR5749609_2_fastqc.html
│   ├── ERR5749609_2_fastqc.zip
│   ├── ERR5749609_fastq_name.csv
│   ├── ERR5771988_1_fastqc.html
│   ├── ERR5771988_1_fastqc.zip
│   ├── ERR5771988_2_fastqc.html
│   ├── ERR5771988_2_fastqc.zip
│   ├── ERR5771988_fastq_name.csv
│   ├── SRR13422799_1_fastqc.html
│   ├── SRR13422799_1_fastqc.zip
│   ├── SRR13422799_2_fastqc.html
│   ├── SRR13422799_2_fastqc.zip
│   ├── SRR13422799_fastq_name.csv
│   ├── SRR13422802_1_fastqc.html
│   ├── SRR13422802_1_fastqc.zip
│   ├── SRR13422802_2_fastqc.html
│   ├── SRR13422802_2_fastqc.zip
│   ├── SRR13422802_fastq_name.csv
│   ├── SRR13500958_1_fastqc.html
│   ├── SRR13500958_1_fastqc.zip
│   ├── SRR13500958_2_fastqc.html
│   ├── SRR13500958_2_fastqc.zip
│   ├── SRR13500958_fastq_name.csv
│   ├── SRR13511054_1_fastqc.html
│   ├── SRR13511054_1_fastqc.zip
│   ├── SRR13511054_2_fastqc.html
│   ├── SRR13511054_2_fastqc.zip
│   ├── SRR13511054_fastq_name.csv
│   ├── SRR13603869_1_fastqc.html
│   ├── SRR13603869_1_fastqc.zip
│   ├── SRR13603869_2_fastqc.html
│   ├── SRR13603869_2_fastqc.zip
│   ├── SRR13603869_fastq_name.csv
│   ├── SRR13606410_1_fastqc.html
│   ├── SRR13606410_1_fastqc.zip
│   ├── SRR13606410_2_fastqc.html
│   ├── SRR13606410_2_fastqc.zip
│   ├── SRR13606410_fastq_name.csv
│   ├── SRR14241199_1_fastqc.html
│   ├── SRR14241199_1_fastqc.zip
│   ├── SRR14241199_2_fastqc.html
│   ├── SRR14241199_2_fastqc.zip
│   └── SRR14241199_fastq_name.csv
├── freyja
│   ├── aggregated-freyja.png
│   ├── aggregated-freyja.tsv
│   ├── ERR5181310_demix.tsv
│   ├── ERR5181310_depths.tsv
│   ├── ERR5181310_freyja_lineages_mqc.png
│   ├── ERR5181310_freyja_lineages.png
│   ├── ERR5181310_variants.tsv
│   ├── ERR5181360_demix.tsv
│   ├── ERR5181360_depths.tsv
│   ├── ERR5181360_freyja_lineages_mqc.png
│   ├── ERR5181360_freyja_lineages.png
│   ├── ERR5181360_variants.tsv
│   ├── ERR5405022_demix.tsv
│   ├── ERR5405022_depths.tsv
│   ├── ERR5405022_freyja_lineages_mqc.png
│   ├── ERR5405022_freyja_lineages.png
│   ├── ERR5405022_variants.tsv
│   ├── ERR5556343_demix.tsv
│   ├── ERR5556343_depths.tsv
│   ├── ERR5556343_freyja_lineages_mqc.png
│   ├── ERR5556343_freyja_lineages.png
│   ├── ERR5556343_variants.tsv
│   ├── ERR5691945_demix.tsv
│   ├── ERR5691945_depths.tsv
│   ├── ERR5691945_freyja_lineages_mqc.png
│   ├── ERR5691945_freyja_lineages.png
│   ├── ERR5691945_variants.tsv
│   ├── ERR5743043_demix.tsv
│   ├── ERR5743043_depths.tsv
│   ├── ERR5743043_freyja_lineages_mqc.png
│   ├── ERR5743043_freyja_lineages.png
│   ├── ERR5743043_variants.tsv
│   ├── ERR5743893_demix.tsv
│   ├── ERR5743893_depths.tsv
│   ├── ERR5743893_freyja_lineages_mqc.png
│   ├── ERR5743893_freyja_lineages.png
│   ├── ERR5743893_variants.tsv
│   ├── ERR5749609_demix.tsv
│   ├── ERR5749609_depths.tsv
│   ├── ERR5749609_freyja_lineages_mqc.png
│   ├── ERR5749609_freyja_lineages.png
│   ├── ERR5749609_variants.tsv
│   ├── ERR5771988_demix.tsv
│   ├── ERR5771988_depths.tsv
│   ├── ERR5771988_freyja_lineages_mqc.png
│   ├── ERR5771988_freyja_lineages.png
│   ├── ERR5771988_variants.tsv
│   ├── SRR13422799_demix.tsv
│   ├── SRR13422799_depths.tsv
│   ├── SRR13422799_freyja_lineages_mqc.png
│   ├── SRR13422799_freyja_lineages.png
│   ├── SRR13422799_variants.tsv
│   ├── SRR13422802_demix.tsv
│   ├── SRR13422802_depths.tsv
│   ├── SRR13422802_freyja_lineages_mqc.png
│   ├── SRR13422802_freyja_lineages.png
│   ├── SRR13422802_variants.tsv
│   ├── SRR13500958_demix.tsv
│   ├── SRR13500958_depths.tsv
│   ├── SRR13500958_freyja_lineages_mqc.png
│   ├── SRR13500958_freyja_lineages.png
│   ├── SRR13500958_variants.tsv
│   ├── SRR13511054_demix.tsv
│   ├── SRR13511054_depths.tsv
│   ├── SRR13511054_freyja_lineages_mqc.png
│   ├── SRR13511054_freyja_lineages.png
│   ├── SRR13511054_variants.tsv
│   ├── SRR13603869_demix.tsv
│   ├── SRR13603869_depths.tsv
│   ├── SRR13603869_freyja_lineages_mqc.png
│   ├── SRR13603869_freyja_lineages.png
│   ├── SRR13603869_variants.tsv
│   ├── SRR13606410_demix.tsv
│   ├── SRR13606410_depths.tsv
│   ├── SRR13606410_freyja_lineages_mqc.png
│   ├── SRR13606410_freyja_lineages.png
│   ├── SRR13606410_variants.tsv
│   ├── SRR14241199_demix.tsv
│   ├── SRR14241199_depths.tsv
│   ├── SRR14241199_freyja_lineages_mqc.png
│   ├── SRR14241199_freyja_lineages.png
│   └── SRR14241199_variants.tsv
├── heatcluster
│   ├── heatcluster.png
│   └── sorted_matrix.csv
├── iqtree2
│   ├── iqtree2.iqtree
│   ├── iqtree2.log
│   ├── iqtree2.mldist
│   ├── iqtree2.treefile
│   └── iqtree2.treefile.nwk
├── ivar_consensus
│   ├── ERR5181310.consensus.fa
│   ├── ERR5181310.consensus.qual.txt
│   ├── ERR5181360.consensus.fa
│   ├── ERR5181360.consensus.qual.txt
│   ├── ERR5405022.consensus.fa
│   ├── ERR5405022.consensus.qual.txt
│   ├── ERR5556343.consensus.fa
│   ├── ERR5556343.consensus.qual.txt
│   ├── ERR5691945.consensus.fa
│   ├── ERR5691945.consensus.qual.txt
│   ├── ERR5743043.consensus.fa
│   ├── ERR5743043.consensus.qual.txt
│   ├── ERR5743893.consensus.fa
│   ├── ERR5743893.consensus.qual.txt
│   ├── ERR5749609.consensus.fa
│   ├── ERR5749609.consensus.qual.txt
│   ├── ERR5771988.consensus.fa
│   ├── ERR5771988.consensus.qual.txt
│   ├── SRR13422799.consensus.fa
│   ├── SRR13422799.consensus.qual.txt
│   ├── SRR13422802.consensus.fa
│   ├── SRR13422802.consensus.qual.txt
│   ├── SRR13500958.consensus.fa
│   ├── SRR13500958.consensus.qual.txt
│   ├── SRR13511054.consensus.fa
│   ├── SRR13511054.consensus.qual.txt
│   ├── SRR13603869.consensus.fa
│   ├── SRR13603869.consensus.qual.txt
│   ├── SRR13606410.consensus.fa
│   ├── SRR13606410.consensus.qual.txt
│   ├── SRR14241199.consensus.fa
│   └── SRR14241199.consensus.qual.txt
├── ivar_trim
│   ├── ERR5181310_ivar.log
│   ├── ERR5181310.primertrim.sorted.bam
│   ├── ERR5181310.primertrim.sorted.bam.bai
│   ├── ERR5181360_ivar.log
│   ├── ERR5181360.primertrim.sorted.bam
│   ├── ERR5181360.primertrim.sorted.bam.bai
│   ├── ERR5405022_ivar.log
│   ├── ERR5405022.primertrim.sorted.bam
│   ├── ERR5405022.primertrim.sorted.bam.bai
│   ├── ERR5556343_ivar.log
│   ├── ERR5556343.primertrim.sorted.bam
│   ├── ERR5556343.primertrim.sorted.bam.bai
│   ├── ERR5691945_ivar.log
│   ├── ERR5691945.primertrim.sorted.bam
│   ├── ERR5691945.primertrim.sorted.bam.bai
│   ├── ERR5743043_ivar.log
│   ├── ERR5743043.primertrim.sorted.bam
│   ├── ERR5743043.primertrim.sorted.bam.bai
│   ├── ERR5743893_ivar.log
│   ├── ERR5743893.primertrim.sorted.bam
│   ├── ERR5743893.primertrim.sorted.bam.bai
│   ├── ERR5749609_ivar.log
│   ├── ERR5749609.primertrim.sorted.bam
│   ├── ERR5749609.primertrim.sorted.bam.bai
│   ├── ERR5771988_ivar.log
│   ├── ERR5771988.primertrim.sorted.bam
│   ├── ERR5771988.primertrim.sorted.bam.bai
│   ├── SRR13422799_ivar.log
│   ├── SRR13422799.primertrim.sorted.bam
│   ├── SRR13422799.primertrim.sorted.bam.bai
│   ├── SRR13422802_ivar.log
│   ├── SRR13422802.primertrim.sorted.bam
│   ├── SRR13422802.primertrim.sorted.bam.bai
│   ├── SRR13500958_ivar.log
│   ├── SRR13500958.primertrim.sorted.bam
│   ├── SRR13500958.primertrim.sorted.bam.bai
│   ├── SRR13511054_ivar.log
│   ├── SRR13511054.primertrim.sorted.bam
│   ├── SRR13511054.primertrim.sorted.bam.bai
│   ├── SRR13603869_ivar.log
│   ├── SRR13603869.primertrim.sorted.bam
│   ├── SRR13603869.primertrim.sorted.bam.bai
│   ├── SRR13606410_ivar.log
│   ├── SRR13606410.primertrim.sorted.bam
│   ├── SRR13606410.primertrim.sorted.bam.bai
│   ├── SRR14241199_ivar.log
│   ├── SRR14241199.primertrim.sorted.bam
│   └── SRR14241199.primertrim.sorted.bam.bai
├── logs
│   └── CECRET:SUBWORKFLOW:PROCESS
│       └── CECRET:SUBWORKFLOW:PROCESS.*.log
├── mafft
│   ├── mafft_aligned.fasta
│   └── ultimate.fasta
├── multiqc
│   ├── multiqc_data
│   │   ├── fastqc_adapter_content_plot.txt
│   │   ├── fastqc_overrepresented_sequences_plot.txt
│   │   ├── fastqc_per_base_n_content_plot.txt
│   │   ├── fastqc_per_base_sequence_quality_plot.txt
│   │   ├── fastqc_per_sequence_gc_content_plot_Counts.txt
│   │   ├── fastqc_per_sequence_gc_content_plot_Percentages.txt
│   │   ├── fastqc_per_sequence_quality_scores_plot.txt
│   │   ├── fastqc_sequence_counts_plot.txt
│   │   ├── fastqc_sequence_duplication_levels_plot.txt
│   │   ├── fastqc_sequence_length_distribution_plot.txt
│   │   ├── fastqc-status-check-heatmap.txt
│   │   ├── fastqc_top_overrepresented_sequences_table.txt
│   │   ├── multiqc_citations.txt
│   │   ├── multiqc_data.json
│   │   ├── multiqc_fastqc.txt
│   │   ├── multiqc_general_stats.txt
│   │   ├── multiqc.log
│   │   ├── multiqc_nextclade.txt
│   │   ├── multiqc_pangolin.txt
│   │   ├── multiqc_samtools_flagstat.txt
│   │   ├── multiqc_samtools_stats.txt
│   │   ├── multiqc_seqyclean.txt
│   │   ├── multiqc_software_versions.txt
│   │   ├── multiqc_sources.txt
│   │   ├── nextclade_run_table.txt
│   │   ├── pangolin_run_table.txt
│   │   ├── samtools_alignment_plot.txt
│   │   ├── samtools-flagstat-dp.txt
│   │   ├── samtools-stats-dp.txt
│   │   ├── seqyclean-discarded-reads-plot.txt
│   │   ├── seqyclean-read-annotation-plot.txt
│   │   └── seqyclean-summary-plot.txt
│   └── multiqc_report.html
├── nextclade
│   ├── combined.fasta
│   ├── nextclade.aligned.fasta
│   ├── nextclade.auspice.json
│   ├── nextclade.cds_translation.E.fasta
│   ├── nextclade.cds_translation.M.fasta
│   ├── nextclade.cds_translation.N.fasta
│   ├── nextclade.cds_translation.ORF1a.fasta
│   ├── nextclade.cds_translation.ORF1b.fasta
│   ├── nextclade.cds_translation.ORF3a.fasta
│   ├── nextclade.cds_translation.ORF6.fasta
│   ├── nextclade.cds_translation.ORF7a.fasta
│   ├── nextclade.cds_translation.ORF7b.fasta
│   ├── nextclade.cds_translation.ORF8.fasta
│   ├── nextclade.cds_translation.ORF9b.fasta
│   ├── nextclade.cds_translation.S.fasta
│   ├── nextclade.csv
│   ├── nextclade.gff
│   ├── nextclade.json
│   ├── nextclade.ndjson
│   ├── nextclade.nwk
│   ├── nextclade.tbl
│   └── nextclade.tsv
├── pango_aliasor
│   └── pango_aliasor.csv
├── pangolin
│   ├── combined.fasta
│   └── lineage_report.csv
├── phytreeviz
│   └── tree.png
├── pipeline_info
│   ├── execution_report_2025-06-24_10-25-21.html
│   ├── execution_report_2025-06-24_10-29-25.html
│   ├── execution_report_2025-06-24_10-50-33.html
│   ├── execution_report_2025-06-24_11-33-11.html
│   ├── execution_timeline_2025-06-24_10-25-21.html
│   ├── execution_timeline_2025-06-24_10-29-25.html
│   ├── execution_timeline_2025-06-24_10-50-33.html
│   ├── execution_timeline_2025-06-24_11-33-11.html
│   ├── execution_trace_2025-06-24_10-25-21.txt
│   ├── execution_trace_2025-06-24_10-29-25.txt
│   ├── execution_trace_2025-06-24_10-49-58.txt
│   ├── execution_trace_2025-06-24_10-50-33.txt
│   ├── execution_trace_2025-06-24_11-33-11.txt
│   ├── pipeline_dag_2025-06-24_10-25-21.html
│   ├── pipeline_dag_2025-06-24_10-29-25.html
│   ├── pipeline_dag_2025-06-24_10-50-33.html
│   └── pipeline_dag_2025-06-24_11-33-11.html
├── samtools
│   ├── ERR5181310_ampliconstats.txt
│   ├── ERR5181310.final.cov.hist
│   ├── ERR5181310.final.cov.txt
│   ├── ERR5181310.final.depth.txt
│   ├── ERR5181310.final.flagstat.txt
│   ├── ERR5181310.final.stats.txt
│   ├── ERR5181310.initial.cov.hist
│   ├── ERR5181310.initial.cov.txt
│   ├── ERR5181310.initial.depth.txt
│   ├── ERR5181310.initial.flagstat.txt
│   ├── ERR5181310.initial.stats.txt
│   ├── ERR5181360_ampliconstats.txt
│   ├── ERR5181360.final.cov.hist
│   ├── ERR5181360.final.cov.txt
│   ├── ERR5181360.final.depth.txt
│   ├── ERR5181360.final.flagstat.txt
│   ├── ERR5181360.final.stats.txt
│   ├── ERR5181360.initial.cov.hist
│   ├── ERR5181360.initial.cov.txt
│   ├── ERR5181360.initial.depth.txt
│   ├── ERR5181360.initial.flagstat.txt
│   ├── ERR5181360.initial.stats.txt
│   ├── ERR5405022_ampliconstats.txt
│   ├── ERR5405022.final.cov.hist
│   ├── ERR5405022.final.cov.txt
│   ├── ERR5405022.final.depth.txt
│   ├── ERR5405022.final.flagstat.txt
│   ├── ERR5405022.final.stats.txt
│   ├── ERR5405022.initial.cov.hist
│   ├── ERR5405022.initial.cov.txt
│   ├── ERR5405022.initial.depth.txt
│   ├── ERR5405022.initial.flagstat.txt
│   ├── ERR5405022.initial.stats.txt
│   ├── ERR5556343_ampliconstats.txt
│   ├── ERR5556343.final.cov.hist
│   ├── ERR5556343.final.cov.txt
│   ├── ERR5556343.final.depth.txt
│   ├── ERR5556343.final.flagstat.txt
│   ├── ERR5556343.final.stats.txt
│   ├── ERR5556343.initial.cov.hist
│   ├── ERR5556343.initial.cov.txt
│   ├── ERR5556343.initial.depth.txt
│   ├── ERR5556343.initial.flagstat.txt
│   ├── ERR5556343.initial.stats.txt
│   ├── ERR5691945_ampliconstats.txt
│   ├── ERR5691945.final.cov.hist
│   ├── ERR5691945.final.cov.txt
│   ├── ERR5691945.final.depth.txt
│   ├── ERR5691945.final.flagstat.txt
│   ├── ERR5691945.final.stats.txt
│   ├── ERR5691945.initial.cov.hist
│   ├── ERR5691945.initial.cov.txt
│   ├── ERR5691945.initial.depth.txt
│   ├── ERR5691945.initial.flagstat.txt
│   ├── ERR5691945.initial.stats.txt
│   ├── ERR5743043_ampliconstats.txt
│   ├── ERR5743043.final.cov.hist
│   ├── ERR5743043.final.cov.txt
│   ├── ERR5743043.final.depth.txt
│   ├── ERR5743043.final.flagstat.txt
│   ├── ERR5743043.final.stats.txt
│   ├── ERR5743043.initial.cov.hist
│   ├── ERR5743043.initial.cov.txt
│   ├── ERR5743043.initial.depth.txt
│   ├── ERR5743043.initial.flagstat.txt
│   ├── ERR5743043.initial.stats.txt
│   ├── ERR5743893_ampliconstats.txt
│   ├── ERR5743893.final.cov.hist
│   ├── ERR5743893.final.cov.txt
│   ├── ERR5743893.final.depth.txt
│   ├── ERR5743893.final.flagstat.txt
│   ├── ERR5743893.final.stats.txt
│   ├── ERR5743893.initial.cov.hist
│   ├── ERR5743893.initial.cov.txt
│   ├── ERR5743893.initial.depth.txt
│   ├── ERR5743893.initial.flagstat.txt
│   ├── ERR5743893.initial.stats.txt
│   ├── ERR5749609_ampliconstats.txt
│   ├── ERR5749609.final.cov.hist
│   ├── ERR5749609.final.cov.txt
│   ├── ERR5749609.final.depth.txt
│   ├── ERR5749609.final.flagstat.txt
│   ├── ERR5749609.final.stats.txt
│   ├── ERR5749609.initial.cov.hist
│   ├── ERR5749609.initial.cov.txt
│   ├── ERR5749609.initial.depth.txt
│   ├── ERR5749609.initial.flagstat.txt
│   ├── ERR5749609.initial.stats.txt
│   ├── ERR5771988_ampliconstats.txt
│   ├── ERR5771988.final.cov.hist
│   ├── ERR5771988.final.cov.txt
│   ├── ERR5771988.final.depth.txt
│   ├── ERR5771988.final.flagstat.txt
│   ├── ERR5771988.final.stats.txt
│   ├── ERR5771988.initial.cov.hist
│   ├── ERR5771988.initial.cov.txt
│   ├── ERR5771988.initial.depth.txt
│   ├── ERR5771988.initial.flagstat.txt
│   ├── ERR5771988.initial.stats.txt
│   ├── final_samtools_coverage_summary.tsv
│   ├── SRR13422799_ampliconstats.txt
│   ├── SRR13422799.final.cov.hist
│   ├── SRR13422799.final.cov.txt
│   ├── SRR13422799.final.depth.txt
│   ├── SRR13422799.final.flagstat.txt
│   ├── SRR13422799.final.stats.txt
│   ├── SRR13422799.initial.cov.hist
│   ├── SRR13422799.initial.cov.txt
│   ├── SRR13422799.initial.depth.txt
│   ├── SRR13422799.initial.flagstat.txt
│   ├── SRR13422799.initial.stats.txt
│   ├── SRR13422802_ampliconstats.txt
│   ├── SRR13422802.final.cov.hist
│   ├── SRR13422802.final.cov.txt
│   ├── SRR13422802.final.depth.txt
│   ├── SRR13422802.final.flagstat.txt
│   ├── SRR13422802.final.stats.txt
│   ├── SRR13422802.initial.cov.hist
│   ├── SRR13422802.initial.cov.txt
│   ├── SRR13422802.initial.depth.txt
│   ├── SRR13422802.initial.flagstat.txt
│   ├── SRR13422802.initial.stats.txt
│   ├── SRR13500958_ampliconstats.txt
│   ├── SRR13500958.final.cov.hist
│   ├── SRR13500958.final.cov.txt
│   ├── SRR13500958.final.depth.txt
│   ├── SRR13500958.final.flagstat.txt
│   ├── SRR13500958.final.stats.txt
│   ├── SRR13500958.initial.cov.hist
│   ├── SRR13500958.initial.cov.txt
│   ├── SRR13500958.initial.depth.txt
│   ├── SRR13500958.initial.flagstat.txt
│   ├── SRR13500958.initial.stats.txt
│   ├── SRR13511054_ampliconstats.txt
│   ├── SRR13511054.final.cov.hist
│   ├── SRR13511054.final.cov.txt
│   ├── SRR13511054.final.depth.txt
│   ├── SRR13511054.final.flagstat.txt
│   ├── SRR13511054.final.stats.txt
│   ├── SRR13511054.initial.cov.hist
│   ├── SRR13511054.initial.cov.txt
│   ├── SRR13511054.initial.depth.txt
│   ├── SRR13511054.initial.flagstat.txt
│   ├── SRR13511054.initial.stats.txt
│   ├── SRR13603869_ampliconstats.txt
│   ├── SRR13603869.final.cov.hist
│   ├── SRR13603869.final.cov.txt
│   ├── SRR13603869.final.depth.txt
│   ├── SRR13603869.final.flagstat.txt
│   ├── SRR13603869.final.stats.txt
│   ├── SRR13603869.initial.cov.hist
│   ├── SRR13603869.initial.cov.txt
│   ├── SRR13603869.initial.depth.txt
│   ├── SRR13603869.initial.flagstat.txt
│   ├── SRR13603869.initial.stats.txt
│   ├── SRR13606410_ampliconstats.txt
│   ├── SRR13606410.final.cov.hist
│   ├── SRR13606410.final.cov.txt
│   ├── SRR13606410.final.depth.txt
│   ├── SRR13606410.final.flagstat.txt
│   ├── SRR13606410.final.stats.txt
│   ├── SRR13606410.initial.cov.hist
│   ├── SRR13606410.initial.cov.txt
│   ├── SRR13606410.initial.depth.txt
│   ├── SRR13606410.initial.flagstat.txt
│   ├── SRR13606410.initial.stats.txt
│   ├── SRR14241199_ampliconstats.txt
│   ├── SRR14241199.final.cov.hist
│   ├── SRR14241199.final.cov.txt
│   ├── SRR14241199.final.depth.txt
│   ├── SRR14241199.final.flagstat.txt
│   ├── SRR14241199.final.stats.txt
│   ├── SRR14241199.initial.cov.hist
│   ├── SRR14241199.initial.cov.txt
│   ├── SRR14241199.initial.depth.txt
│   ├── SRR14241199.initial.flagstat.txt
│   └── SRR14241199.initial.stats.txt
├── samtools_plot_ampliconstats
│   ├── ERR5181310
│   ├── ERR5181310-combined-amp.gp
│   ├── ERR5181310-combined-amp.png
│   ├── ERR5181310-combined-coverage-1.gp
│   ├── ERR5181310-combined-coverage-1.png
│   ├── ERR5181310-combined-depth.gp
│   ├── ERR5181310-combined-depth.png
│   ├── ERR5181310-combined-read-perc.gp
│   ├── ERR5181310-combined-read-perc.png
│   ├── ERR5181310-combined-reads.gp
│   ├── ERR5181310-combined-reads.png
│   ├── ERR5181310-combined-tcoord.gp
│   ├── ERR5181310-combined-tcoord.png
│   ├── ERR5181310-combined-tdepth.gp
│   ├── ERR5181310-combined-tdepth.png
│   ├── ERR5181310-ERR5181310.primertrim.sorted-amp.gp
│   ├── ERR5181310-ERR5181310.primertrim.sorted-amp.png
│   ├── ERR5181310-ERR5181310.primertrim.sorted-cov.gp
│   ├── ERR5181310-ERR5181310.primertrim.sorted-cov.png
│   ├── ERR5181310-ERR5181310.primertrim.sorted-reads.gp
│   ├── ERR5181310-ERR5181310.primertrim.sorted-reads.png
│   ├── ERR5181310-ERR5181310.primertrim.sorted-tcoord.gp
│   ├── ERR5181310-ERR5181310.primertrim.sorted-tcoord.png
│   ├── ERR5181310-ERR5181310.primertrim.sorted-tdepth.gp
│   ├── ERR5181310-ERR5181310.primertrim.sorted-tdepth.png
│   ├── ERR5181310-ERR5181310.primertrim.sorted-tsize.gp
│   ├── ERR5181310-ERR5181310.primertrim.sorted-tsize.png
│   ├── ERR5181310-heat-amp-1.gp
│   ├── ERR5181310-heat-amp-1.png
│   ├── ERR5181310-heat-coverage-1-1.gp
│   ├── ERR5181310-heat-coverage-1-1.png
│   ├── ERR5181310-heat-read-perc-1.gp
│   ├── ERR5181310-heat-read-perc-1.png
│   ├── ERR5181310-heat-read-perc-log-1.gp
│   ├── ERR5181310-heat-read-perc-log-1.png
│   ├── ERR5181310-heat-reads-1.gp
│   ├── ERR5181310-heat-reads-1.png
│   ├── ERR5181360
│   ├── ERR5181360-combined-amp.gp
│   ├── ERR5181360-combined-amp.png
│   ├── ERR5181360-combined-coverage-1.gp
│   ├── ERR5181360-combined-coverage-1.png
│   ├── ERR5181360-combined-depth.gp
│   ├── ERR5181360-combined-depth.png
│   ├── ERR5181360-combined-read-perc.gp
│   ├── ERR5181360-combined-read-perc.png
│   ├── ERR5181360-combined-reads.gp
│   ├── ERR5181360-combined-reads.png
│   ├── ERR5181360-combined-tcoord.gp
│   ├── ERR5181360-combined-tcoord.png
│   ├── ERR5181360-combined-tdepth.gp
│   ├── ERR5181360-combined-tdepth.png
│   ├── ERR5181360-ERR5181360.primertrim.sorted-amp.gp
│   ├── ERR5181360-ERR5181360.primertrim.sorted-amp.png
│   ├── ERR5181360-ERR5181360.primertrim.sorted-cov.gp
│   ├── ERR5181360-ERR5181360.primertrim.sorted-cov.png
│   ├── ERR5181360-ERR5181360.primertrim.sorted-reads.gp
│   ├── ERR5181360-ERR5181360.primertrim.sorted-reads.png
│   ├── ERR5181360-ERR5181360.primertrim.sorted-tcoord.gp
│   ├── ERR5181360-ERR5181360.primertrim.sorted-tcoord.png
│   ├── ERR5181360-ERR5181360.primertrim.sorted-tdepth.gp
│   ├── ERR5181360-ERR5181360.primertrim.sorted-tdepth.png
│   ├── ERR5181360-ERR5181360.primertrim.sorted-tsize.gp
│   ├── ERR5181360-ERR5181360.primertrim.sorted-tsize.png
│   ├── ERR5181360-heat-amp-1.gp
│   ├── ERR5181360-heat-amp-1.png
│   ├── ERR5181360-heat-coverage-1-1.gp
│   ├── ERR5181360-heat-coverage-1-1.png
│   ├── ERR5181360-heat-read-perc-1.gp
│   ├── ERR5181360-heat-read-perc-1.png
│   ├── ERR5181360-heat-read-perc-log-1.gp
│   ├── ERR5181360-heat-read-perc-log-1.png
│   ├── ERR5181360-heat-reads-1.gp
│   ├── ERR5181360-heat-reads-1.png
│   ├── ERR5405022
│   ├── ERR5405022-combined-amp.gp
│   ├── ERR5405022-combined-amp.png
│   ├── ERR5405022-combined-coverage-1.gp
│   ├── ERR5405022-combined-coverage-1.png
│   ├── ERR5405022-combined-depth.gp
│   ├── ERR5405022-combined-depth.png
│   ├── ERR5405022-combined-read-perc.gp
│   ├── ERR5405022-combined-read-perc.png
│   ├── ERR5405022-combined-reads.gp
│   ├── ERR5405022-combined-reads.png
│   ├── ERR5405022-combined-tcoord.gp
│   ├── ERR5405022-combined-tcoord.png
│   ├── ERR5405022-combined-tdepth.gp
│   ├── ERR5405022-combined-tdepth.png
│   ├── ERR5405022-ERR5405022.primertrim.sorted-amp.gp
│   ├── ERR5405022-ERR5405022.primertrim.sorted-amp.png
│   ├── ERR5405022-ERR5405022.primertrim.sorted-cov.gp
│   ├── ERR5405022-ERR5405022.primertrim.sorted-cov.png
│   ├── ERR5405022-ERR5405022.primertrim.sorted-reads.gp
│   ├── ERR5405022-ERR5405022.primertrim.sorted-reads.png
│   ├── ERR5405022-ERR5405022.primertrim.sorted-tcoord.gp
│   ├── ERR5405022-ERR5405022.primertrim.sorted-tcoord.png
│   ├── ERR5405022-ERR5405022.primertrim.sorted-tdepth.gp
│   ├── ERR5405022-ERR5405022.primertrim.sorted-tdepth.png
│   ├── ERR5405022-ERR5405022.primertrim.sorted-tsize.gp
│   ├── ERR5405022-ERR5405022.primertrim.sorted-tsize.png
│   ├── ERR5405022-heat-amp-1.gp
│   ├── ERR5405022-heat-amp-1.png
│   ├── ERR5405022-heat-coverage-1-1.gp
│   ├── ERR5405022-heat-coverage-1-1.png
│   ├── ERR5405022-heat-read-perc-1.gp
│   ├── ERR5405022-heat-read-perc-1.png
│   ├── ERR5405022-heat-read-perc-log-1.gp
│   ├── ERR5405022-heat-read-perc-log-1.png
│   ├── ERR5405022-heat-reads-1.gp
│   ├── ERR5405022-heat-reads-1.png
│   ├── ERR5556343
│   ├── ERR5556343-combined-amp.gp
│   ├── ERR5556343-combined-amp.png
│   ├── ERR5556343-combined-coverage-1.gp
│   ├── ERR5556343-combined-coverage-1.png
│   ├── ERR5556343-combined-depth.gp
│   ├── ERR5556343-combined-depth.png
│   ├── ERR5556343-combined-read-perc.gp
│   ├── ERR5556343-combined-read-perc.png
│   ├── ERR5556343-combined-reads.gp
│   ├── ERR5556343-combined-reads.png
│   ├── ERR5556343-combined-tcoord.gp
│   ├── ERR5556343-combined-tcoord.png
│   ├── ERR5556343-combined-tdepth.gp
│   ├── ERR5556343-combined-tdepth.png
│   ├── ERR5556343-ERR5556343.primertrim.sorted-amp.gp
│   ├── ERR5556343-ERR5556343.primertrim.sorted-amp.png
│   ├── ERR5556343-ERR5556343.primertrim.sorted-cov.gp
│   ├── ERR5556343-ERR5556343.primertrim.sorted-cov.png
│   ├── ERR5556343-ERR5556343.primertrim.sorted-reads.gp
│   ├── ERR5556343-ERR5556343.primertrim.sorted-reads.png
│   ├── ERR5556343-ERR5556343.primertrim.sorted-tcoord.gp
│   ├── ERR5556343-ERR5556343.primertrim.sorted-tcoord.png
│   ├── ERR5556343-ERR5556343.primertrim.sorted-tdepth.gp
│   ├── ERR5556343-ERR5556343.primertrim.sorted-tdepth.png
│   ├── ERR5556343-ERR5556343.primertrim.sorted-tsize.gp
│   ├── ERR5556343-ERR5556343.primertrim.sorted-tsize.png
│   ├── ERR5556343-heat-amp-1.gp
│   ├── ERR5556343-heat-amp-1.png
│   ├── ERR5556343-heat-coverage-1-1.gp
│   ├── ERR5556343-heat-coverage-1-1.png
│   ├── ERR5556343-heat-read-perc-1.gp
│   ├── ERR5556343-heat-read-perc-1.png
│   ├── ERR5556343-heat-read-perc-log-1.gp
│   ├── ERR5556343-heat-read-perc-log-1.png
│   ├── ERR5556343-heat-reads-1.gp
│   ├── ERR5556343-heat-reads-1.png
│   ├── ERR5691945
│   ├── ERR5691945-combined-amp.gp
│   ├── ERR5691945-combined-amp.png
│   ├── ERR5691945-combined-coverage-1.gp
│   ├── ERR5691945-combined-coverage-1.png
│   ├── ERR5691945-combined-depth.gp
│   ├── ERR5691945-combined-depth.png
│   ├── ERR5691945-combined-read-perc.gp
│   ├── ERR5691945-combined-read-perc.png
│   ├── ERR5691945-combined-reads.gp
│   ├── ERR5691945-combined-reads.png
│   ├── ERR5691945-combined-tcoord.gp
│   ├── ERR5691945-combined-tcoord.png
│   ├── ERR5691945-combined-tdepth.gp
│   ├── ERR5691945-combined-tdepth.png
│   ├── ERR5691945-ERR5691945.primertrim.sorted-amp.gp
│   ├── ERR5691945-ERR5691945.primertrim.sorted-amp.png
│   ├── ERR5691945-ERR5691945.primertrim.sorted-cov.gp
│   ├── ERR5691945-ERR5691945.primertrim.sorted-cov.png
│   ├── ERR5691945-ERR5691945.primertrim.sorted-reads.gp
│   ├── ERR5691945-ERR5691945.primertrim.sorted-reads.png
│   ├── ERR5691945-ERR5691945.primertrim.sorted-tcoord.gp
│   ├── ERR5691945-ERR5691945.primertrim.sorted-tcoord.png
│   ├── ERR5691945-ERR5691945.primertrim.sorted-tdepth.gp
│   ├── ERR5691945-ERR5691945.primertrim.sorted-tdepth.png
│   ├── ERR5691945-ERR5691945.primertrim.sorted-tsize.gp
│   ├── ERR5691945-ERR5691945.primertrim.sorted-tsize.png
│   ├── ERR5691945-heat-amp-1.gp
│   ├── ERR5691945-heat-amp-1.png
│   ├── ERR5691945-heat-coverage-1-1.gp
│   ├── ERR5691945-heat-coverage-1-1.png
│   ├── ERR5691945-heat-read-perc-1.gp
│   ├── ERR5691945-heat-read-perc-1.png
│   ├── ERR5691945-heat-read-perc-log-1.gp
│   ├── ERR5691945-heat-read-perc-log-1.png
│   ├── ERR5691945-heat-reads-1.gp
│   ├── ERR5691945-heat-reads-1.png
│   ├── ERR5743043
│   ├── ERR5743043-combined-amp.gp
│   ├── ERR5743043-combined-amp.png
│   ├── ERR5743043-combined-coverage-1.gp
│   ├── ERR5743043-combined-coverage-1.png
│   ├── ERR5743043-combined-depth.gp
│   ├── ERR5743043-combined-depth.png
│   ├── ERR5743043-combined-read-perc.gp
│   ├── ERR5743043-combined-read-perc.png
│   ├── ERR5743043-combined-reads.gp
│   ├── ERR5743043-combined-reads.png
│   ├── ERR5743043-combined-tcoord.gp
│   ├── ERR5743043-combined-tcoord.png
│   ├── ERR5743043-combined-tdepth.gp
│   ├── ERR5743043-combined-tdepth.png
│   ├── ERR5743043-ERR5743043.primertrim.sorted-amp.gp
│   ├── ERR5743043-ERR5743043.primertrim.sorted-amp.png
│   ├── ERR5743043-ERR5743043.primertrim.sorted-cov.gp
│   ├── ERR5743043-ERR5743043.primertrim.sorted-cov.png
│   ├── ERR5743043-ERR5743043.primertrim.sorted-reads.gp
│   ├── ERR5743043-ERR5743043.primertrim.sorted-reads.png
│   ├── ERR5743043-ERR5743043.primertrim.sorted-tcoord.gp
│   ├── ERR5743043-ERR5743043.primertrim.sorted-tcoord.png
│   ├── ERR5743043-ERR5743043.primertrim.sorted-tdepth.gp
│   ├── ERR5743043-ERR5743043.primertrim.sorted-tdepth.png
│   ├── ERR5743043-ERR5743043.primertrim.sorted-tsize.gp
│   ├── ERR5743043-ERR5743043.primertrim.sorted-tsize.png
│   ├── ERR5743043-heat-amp-1.gp
│   ├── ERR5743043-heat-amp-1.png
│   ├── ERR5743043-heat-coverage-1-1.gp
│   ├── ERR5743043-heat-coverage-1-1.png
│   ├── ERR5743043-heat-read-perc-1.gp
│   ├── ERR5743043-heat-read-perc-1.png
│   ├── ERR5743043-heat-read-perc-log-1.gp
│   ├── ERR5743043-heat-read-perc-log-1.png
│   ├── ERR5743043-heat-reads-1.gp
│   ├── ERR5743043-heat-reads-1.png
│   ├── ERR5743893
│   ├── ERR5743893-combined-amp.gp
│   ├── ERR5743893-combined-amp.png
│   ├── ERR5743893-combined-coverage-1.gp
│   ├── ERR5743893-combined-coverage-1.png
│   ├── ERR5743893-combined-depth.gp
│   ├── ERR5743893-combined-depth.png
│   ├── ERR5743893-combined-read-perc.gp
│   ├── ERR5743893-combined-read-perc.png
│   ├── ERR5743893-combined-reads.gp
│   ├── ERR5743893-combined-reads.png
│   ├── ERR5743893-combined-tcoord.gp
│   ├── ERR5743893-combined-tcoord.png
│   ├── ERR5743893-combined-tdepth.gp
│   ├── ERR5743893-combined-tdepth.png
│   ├── ERR5743893-ERR5743893.primertrim.sorted-amp.gp
│   ├── ERR5743893-ERR5743893.primertrim.sorted-amp.png
│   ├── ERR5743893-ERR5743893.primertrim.sorted-cov.gp
│   ├── ERR5743893-ERR5743893.primertrim.sorted-cov.png
│   ├── ERR5743893-ERR5743893.primertrim.sorted-reads.gp
│   ├── ERR5743893-ERR5743893.primertrim.sorted-reads.png
│   ├── ERR5743893-ERR5743893.primertrim.sorted-tcoord.gp
│   ├── ERR5743893-ERR5743893.primertrim.sorted-tcoord.png
│   ├── ERR5743893-ERR5743893.primertrim.sorted-tdepth.gp
│   ├── ERR5743893-ERR5743893.primertrim.sorted-tdepth.png
│   ├── ERR5743893-ERR5743893.primertrim.sorted-tsize.gp
│   ├── ERR5743893-ERR5743893.primertrim.sorted-tsize.png
│   ├── ERR5743893-heat-amp-1.gp
│   ├── ERR5743893-heat-amp-1.png
│   ├── ERR5743893-heat-coverage-1-1.gp
│   ├── ERR5743893-heat-coverage-1-1.png
│   ├── ERR5743893-heat-read-perc-1.gp
│   ├── ERR5743893-heat-read-perc-1.png
│   ├── ERR5743893-heat-read-perc-log-1.gp
│   ├── ERR5743893-heat-read-perc-log-1.png
│   ├── ERR5743893-heat-reads-1.gp
│   ├── ERR5743893-heat-reads-1.png
│   ├── ERR5749609
│   ├── ERR5749609-combined-amp.gp
│   ├── ERR5749609-combined-amp.png
│   ├── ERR5749609-combined-coverage-1.gp
│   ├── ERR5749609-combined-coverage-1.png
│   ├── ERR5749609-combined-depth.gp
│   ├── ERR5749609-combined-depth.png
│   ├── ERR5749609-combined-read-perc.gp
│   ├── ERR5749609-combined-read-perc.png
│   ├── ERR5749609-combined-reads.gp
│   ├── ERR5749609-combined-reads.png
│   ├── ERR5749609-combined-tcoord.gp
│   ├── ERR5749609-combined-tcoord.png
│   ├── ERR5749609-combined-tdepth.gp
│   ├── ERR5749609-combined-tdepth.png
│   ├── ERR5749609-ERR5749609.primertrim.sorted-amp.gp
│   ├── ERR5749609-ERR5749609.primertrim.sorted-amp.png
│   ├── ERR5749609-ERR5749609.primertrim.sorted-cov.gp
│   ├── ERR5749609-ERR5749609.primertrim.sorted-cov.png
│   ├── ERR5749609-ERR5749609.primertrim.sorted-reads.gp
│   ├── ERR5749609-ERR5749609.primertrim.sorted-reads.png
│   ├── ERR5749609-ERR5749609.primertrim.sorted-tcoord.gp
│   ├── ERR5749609-ERR5749609.primertrim.sorted-tcoord.png
│   ├── ERR5749609-ERR5749609.primertrim.sorted-tdepth.gp
│   ├── ERR5749609-ERR5749609.primertrim.sorted-tdepth.png
│   ├── ERR5749609-ERR5749609.primertrim.sorted-tsize.gp
│   ├── ERR5749609-ERR5749609.primertrim.sorted-tsize.png
│   ├── ERR5749609-heat-amp-1.gp
│   ├── ERR5749609-heat-amp-1.png
│   ├── ERR5749609-heat-coverage-1-1.gp
│   ├── ERR5749609-heat-coverage-1-1.png
│   ├── ERR5749609-heat-read-perc-1.gp
│   ├── ERR5749609-heat-read-perc-1.png
│   ├── ERR5749609-heat-read-perc-log-1.gp
│   ├── ERR5749609-heat-read-perc-log-1.png
│   ├── ERR5749609-heat-reads-1.gp
│   ├── ERR5749609-heat-reads-1.png
│   ├── ERR5771988
│   ├── ERR5771988-combined-amp.gp
│   ├── ERR5771988-combined-amp.png
│   ├── ERR5771988-combined-coverage-1.gp
│   ├── ERR5771988-combined-coverage-1.png
│   ├── ERR5771988-combined-depth.gp
│   ├── ERR5771988-combined-depth.png
│   ├── ERR5771988-combined-read-perc.gp
│   ├── ERR5771988-combined-read-perc.png
│   ├── ERR5771988-combined-reads.gp
│   ├── ERR5771988-combined-reads.png
│   ├── ERR5771988-combined-tcoord.gp
│   ├── ERR5771988-combined-tcoord.png
│   ├── ERR5771988-combined-tdepth.gp
│   ├── ERR5771988-combined-tdepth.png
│   ├── ERR5771988-ERR5771988.primertrim.sorted-amp.gp
│   ├── ERR5771988-ERR5771988.primertrim.sorted-amp.png
│   ├── ERR5771988-ERR5771988.primertrim.sorted-cov.gp
│   ├── ERR5771988-ERR5771988.primertrim.sorted-cov.png
│   ├── ERR5771988-ERR5771988.primertrim.sorted-reads.gp
│   ├── ERR5771988-ERR5771988.primertrim.sorted-reads.png
│   ├── ERR5771988-ERR5771988.primertrim.sorted-tcoord.gp
│   ├── ERR5771988-ERR5771988.primertrim.sorted-tcoord.png
│   ├── ERR5771988-ERR5771988.primertrim.sorted-tdepth.gp
│   ├── ERR5771988-ERR5771988.primertrim.sorted-tdepth.png
│   ├── ERR5771988-ERR5771988.primertrim.sorted-tsize.gp
│   ├── ERR5771988-ERR5771988.primertrim.sorted-tsize.png
│   ├── ERR5771988-heat-amp-1.gp
│   ├── ERR5771988-heat-amp-1.png
│   ├── ERR5771988-heat-coverage-1-1.gp
│   ├── ERR5771988-heat-coverage-1-1.png
│   ├── ERR5771988-heat-read-perc-1.gp
│   ├── ERR5771988-heat-read-perc-1.png
│   ├── ERR5771988-heat-read-perc-log-1.gp
│   ├── ERR5771988-heat-read-perc-log-1.png
│   ├── ERR5771988-heat-reads-1.gp
│   ├── ERR5771988-heat-reads-1.png
│   ├── index.html
│   ├── SRR13422799
│   ├── SRR13422799-combined-amp.gp
│   ├── SRR13422799-combined-amp.png
│   ├── SRR13422799-combined-coverage-1.gp
│   ├── SRR13422799-combined-coverage-1.png
│   ├── SRR13422799-combined-depth.gp
│   ├── SRR13422799-combined-depth.png
│   ├── SRR13422799-combined-read-perc.gp
│   ├── SRR13422799-combined-read-perc.png
│   ├── SRR13422799-combined-reads.gp
│   ├── SRR13422799-combined-reads.png
│   ├── SRR13422799-combined-tcoord.gp
│   ├── SRR13422799-combined-tcoord.png
│   ├── SRR13422799-combined-tdepth.gp
│   ├── SRR13422799-combined-tdepth.png
│   ├── SRR13422799-heat-amp-1.gp
│   ├── SRR13422799-heat-amp-1.png
│   ├── SRR13422799-heat-coverage-1-1.gp
│   ├── SRR13422799-heat-coverage-1-1.png
│   ├── SRR13422799-heat-read-perc-1.gp
│   ├── SRR13422799-heat-read-perc-1.png
│   ├── SRR13422799-heat-read-perc-log-1.gp
│   ├── SRR13422799-heat-read-perc-log-1.png
│   ├── SRR13422799-heat-reads-1.gp
│   ├── SRR13422799-heat-reads-1.png
│   ├── SRR13422799-SRR13422799.primertrim.sorted-amp.gp
│   ├── SRR13422799-SRR13422799.primertrim.sorted-amp.png
│   ├── SRR13422799-SRR13422799.primertrim.sorted-cov.gp
│   ├── SRR13422799-SRR13422799.primertrim.sorted-cov.png
│   ├── SRR13422799-SRR13422799.primertrim.sorted-reads.gp
│   ├── SRR13422799-SRR13422799.primertrim.sorted-reads.png
│   ├── SRR13422799-SRR13422799.primertrim.sorted-tcoord.gp
│   ├── SRR13422799-SRR13422799.primertrim.sorted-tcoord.png
│   ├── SRR13422799-SRR13422799.primertrim.sorted-tdepth.gp
│   ├── SRR13422799-SRR13422799.primertrim.sorted-tdepth.png
│   ├── SRR13422799-SRR13422799.primertrim.sorted-tsize.gp
│   ├── SRR13422799-SRR13422799.primertrim.sorted-tsize.png
│   ├── SRR13422802
│   ├── SRR13422802-combined-amp.gp
│   ├── SRR13422802-combined-amp.png
│   ├── SRR13422802-combined-coverage-1.gp
│   ├── SRR13422802-combined-coverage-1.png
│   ├── SRR13422802-combined-depth.gp
│   ├── SRR13422802-combined-depth.png
│   ├── SRR13422802-combined-read-perc.gp
│   ├── SRR13422802-combined-read-perc.png
│   ├── SRR13422802-combined-reads.gp
│   ├── SRR13422802-combined-reads.png
│   ├── SRR13422802-combined-tcoord.gp
│   ├── SRR13422802-combined-tcoord.png
│   ├── SRR13422802-combined-tdepth.gp
│   ├── SRR13422802-combined-tdepth.png
│   ├── SRR13422802-heat-amp-1.gp
│   ├── SRR13422802-heat-amp-1.png
│   ├── SRR13422802-heat-coverage-1-1.gp
│   ├── SRR13422802-heat-coverage-1-1.png
│   ├── SRR13422802-heat-read-perc-1.gp
│   ├── SRR13422802-heat-read-perc-1.png
│   ├── SRR13422802-heat-read-perc-log-1.gp
│   ├── SRR13422802-heat-read-perc-log-1.png
│   ├── SRR13422802-heat-reads-1.gp
│   ├── SRR13422802-heat-reads-1.png
│   ├── SRR13422802-SRR13422802.primertrim.sorted-amp.gp
│   ├── SRR13422802-SRR13422802.primertrim.sorted-amp.png
│   ├── SRR13422802-SRR13422802.primertrim.sorted-cov.gp
│   ├── SRR13422802-SRR13422802.primertrim.sorted-cov.png
│   ├── SRR13422802-SRR13422802.primertrim.sorted-reads.gp
│   ├── SRR13422802-SRR13422802.primertrim.sorted-reads.png
│   ├── SRR13422802-SRR13422802.primertrim.sorted-tcoord.gp
│   ├── SRR13422802-SRR13422802.primertrim.sorted-tcoord.png
│   ├── SRR13422802-SRR13422802.primertrim.sorted-tdepth.gp
│   ├── SRR13422802-SRR13422802.primertrim.sorted-tdepth.png
│   ├── SRR13422802-SRR13422802.primertrim.sorted-tsize.gp
│   ├── SRR13422802-SRR13422802.primertrim.sorted-tsize.png
│   ├── SRR13500958
│   ├── SRR13500958-combined-amp.gp
│   ├── SRR13500958-combined-amp.png
│   ├── SRR13500958-combined-coverage-1.gp
│   ├── SRR13500958-combined-coverage-1.png
│   ├── SRR13500958-combined-depth.gp
│   ├── SRR13500958-combined-depth.png
│   ├── SRR13500958-combined-read-perc.gp
│   ├── SRR13500958-combined-read-perc.png
│   ├── SRR13500958-combined-reads.gp
│   ├── SRR13500958-combined-reads.png
│   ├── SRR13500958-combined-tcoord.gp
│   ├── SRR13500958-combined-tcoord.png
│   ├── SRR13500958-combined-tdepth.gp
│   ├── SRR13500958-combined-tdepth.png
│   ├── SRR13500958-heat-amp-1.gp
│   ├── SRR13500958-heat-amp-1.png
│   ├── SRR13500958-heat-coverage-1-1.gp
│   ├── SRR13500958-heat-coverage-1-1.png
│   ├── SRR13500958-heat-read-perc-1.gp
│   ├── SRR13500958-heat-read-perc-1.png
│   ├── SRR13500958-heat-read-perc-log-1.gp
│   ├── SRR13500958-heat-read-perc-log-1.png
│   ├── SRR13500958-heat-reads-1.gp
│   ├── SRR13500958-heat-reads-1.png
│   ├── SRR13500958-SRR13500958.primertrim.sorted-amp.gp
│   ├── SRR13500958-SRR13500958.primertrim.sorted-amp.png
│   ├── SRR13500958-SRR13500958.primertrim.sorted-cov.gp
│   ├── SRR13500958-SRR13500958.primertrim.sorted-cov.png
│   ├── SRR13500958-SRR13500958.primertrim.sorted-reads.gp
│   ├── SRR13500958-SRR13500958.primertrim.sorted-reads.png
│   ├── SRR13500958-SRR13500958.primertrim.sorted-tcoord.gp
│   ├── SRR13500958-SRR13500958.primertrim.sorted-tcoord.png
│   ├── SRR13500958-SRR13500958.primertrim.sorted-tdepth.gp
│   ├── SRR13500958-SRR13500958.primertrim.sorted-tdepth.png
│   ├── SRR13500958-SRR13500958.primertrim.sorted-tsize.gp
│   ├── SRR13500958-SRR13500958.primertrim.sorted-tsize.png
│   ├── SRR13511054
│   ├── SRR13511054-combined-amp.gp
│   ├── SRR13511054-combined-amp.png
│   ├── SRR13511054-combined-coverage-1.gp
│   ├── SRR13511054-combined-coverage-1.png
│   ├── SRR13511054-combined-depth.gp
│   ├── SRR13511054-combined-depth.png
│   ├── SRR13511054-combined-read-perc.gp
│   ├── SRR13511054-combined-read-perc.png
│   ├── SRR13511054-combined-reads.gp
│   ├── SRR13511054-combined-reads.png
│   ├── SRR13511054-combined-tcoord.gp
│   ├── SRR13511054-combined-tcoord.png
│   ├── SRR13511054-combined-tdepth.gp
│   ├── SRR13511054-combined-tdepth.png
│   ├── SRR13511054-heat-amp-1.gp
│   ├── SRR13511054-heat-amp-1.png
│   ├── SRR13511054-heat-coverage-1-1.gp
│   ├── SRR13511054-heat-coverage-1-1.png
│   ├── SRR13511054-heat-read-perc-1.gp
│   ├── SRR13511054-heat-read-perc-1.png
│   ├── SRR13511054-heat-read-perc-log-1.gp
│   ├── SRR13511054-heat-read-perc-log-1.png
│   ├── SRR13511054-heat-reads-1.gp
│   ├── SRR13511054-heat-reads-1.png
│   ├── SRR13511054-SRR13511054.primertrim.sorted-amp.gp
│   ├── SRR13511054-SRR13511054.primertrim.sorted-amp.png
│   ├── SRR13511054-SRR13511054.primertrim.sorted-cov.gp
│   ├── SRR13511054-SRR13511054.primertrim.sorted-cov.png
│   ├── SRR13511054-SRR13511054.primertrim.sorted-reads.gp
│   ├── SRR13511054-SRR13511054.primertrim.sorted-reads.png
│   ├── SRR13511054-SRR13511054.primertrim.sorted-tcoord.gp
│   ├── SRR13511054-SRR13511054.primertrim.sorted-tcoord.png
│   ├── SRR13511054-SRR13511054.primertrim.sorted-tdepth.gp
│   ├── SRR13511054-SRR13511054.primertrim.sorted-tdepth.png
│   ├── SRR13511054-SRR13511054.primertrim.sorted-tsize.gp
│   ├── SRR13511054-SRR13511054.primertrim.sorted-tsize.png
│   ├── SRR13603869
│   ├── SRR13603869-combined-amp.gp
│   ├── SRR13603869-combined-amp.png
│   ├── SRR13603869-combined-coverage-1.gp
│   ├── SRR13603869-combined-coverage-1.png
│   ├── SRR13603869-combined-depth.gp
│   ├── SRR13603869-combined-depth.png
│   ├── SRR13603869-combined-read-perc.gp
│   ├── SRR13603869-combined-read-perc.png
│   ├── SRR13603869-combined-reads.gp
│   ├── SRR13603869-combined-reads.png
│   ├── SRR13603869-combined-tcoord.gp
│   ├── SRR13603869-combined-tcoord.png
│   ├── SRR13603869-combined-tdepth.gp
│   ├── SRR13603869-combined-tdepth.png
│   ├── SRR13603869-heat-amp-1.gp
│   ├── SRR13603869-heat-amp-1.png
│   ├── SRR13603869-heat-coverage-1-1.gp
│   ├── SRR13603869-heat-coverage-1-1.png
│   ├── SRR13603869-heat-read-perc-1.gp
│   ├── SRR13603869-heat-read-perc-1.png
│   ├── SRR13603869-heat-read-perc-log-1.gp
│   ├── SRR13603869-heat-read-perc-log-1.png
│   ├── SRR13603869-heat-reads-1.gp
│   ├── SRR13603869-heat-reads-1.png
│   ├── SRR13603869-SRR13603869.primertrim.sorted-amp.gp
│   ├── SRR13603869-SRR13603869.primertrim.sorted-amp.png
│   ├── SRR13603869-SRR13603869.primertrim.sorted-cov.gp
│   ├── SRR13603869-SRR13603869.primertrim.sorted-cov.png
│   ├── SRR13603869-SRR13603869.primertrim.sorted-reads.gp
│   ├── SRR13603869-SRR13603869.primertrim.sorted-reads.png
│   ├── SRR13603869-SRR13603869.primertrim.sorted-tcoord.gp
│   ├── SRR13603869-SRR13603869.primertrim.sorted-tcoord.png
│   ├── SRR13603869-SRR13603869.primertrim.sorted-tdepth.gp
│   ├── SRR13603869-SRR13603869.primertrim.sorted-tdepth.png
│   ├── SRR13603869-SRR13603869.primertrim.sorted-tsize.gp
│   ├── SRR13603869-SRR13603869.primertrim.sorted-tsize.png
│   ├── SRR13606410
│   ├── SRR13606410-combined-amp.gp
│   ├── SRR13606410-combined-amp.png
│   ├── SRR13606410-combined-coverage-1.gp
│   ├── SRR13606410-combined-coverage-1.png
│   ├── SRR13606410-combined-depth.gp
│   ├── SRR13606410-combined-depth.png
│   ├── SRR13606410-combined-read-perc.gp
│   ├── SRR13606410-combined-read-perc.png
│   ├── SRR13606410-combined-reads.gp
│   ├── SRR13606410-combined-reads.png
│   ├── SRR13606410-combined-tcoord.gp
│   ├── SRR13606410-combined-tcoord.png
│   ├── SRR13606410-combined-tdepth.gp
│   ├── SRR13606410-combined-tdepth.png
│   ├── SRR13606410-heat-amp-1.gp
│   ├── SRR13606410-heat-amp-1.png
│   ├── SRR13606410-heat-coverage-1-1.gp
│   ├── SRR13606410-heat-coverage-1-1.png
│   ├── SRR13606410-heat-read-perc-1.gp
│   ├── SRR13606410-heat-read-perc-1.png
│   ├── SRR13606410-heat-read-perc-log-1.gp
│   ├── SRR13606410-heat-read-perc-log-1.png
│   ├── SRR13606410-heat-reads-1.gp
│   ├── SRR13606410-heat-reads-1.png
│   ├── SRR13606410-SRR13606410.primertrim.sorted-amp.gp
│   ├── SRR13606410-SRR13606410.primertrim.sorted-amp.png
│   ├── SRR13606410-SRR13606410.primertrim.sorted-cov.gp
│   ├── SRR13606410-SRR13606410.primertrim.sorted-cov.png
│   ├── SRR13606410-SRR13606410.primertrim.sorted-reads.gp
│   ├── SRR13606410-SRR13606410.primertrim.sorted-reads.png
│   ├── SRR13606410-SRR13606410.primertrim.sorted-tcoord.gp
│   ├── SRR13606410-SRR13606410.primertrim.sorted-tcoord.png
│   ├── SRR13606410-SRR13606410.primertrim.sorted-tdepth.gp
│   ├── SRR13606410-SRR13606410.primertrim.sorted-tdepth.png
│   ├── SRR13606410-SRR13606410.primertrim.sorted-tsize.gp
│   ├── SRR13606410-SRR13606410.primertrim.sorted-tsize.png
│   ├── SRR14241199
│   ├── SRR14241199-combined-amp.gp
│   ├── SRR14241199-combined-amp.png
│   ├── SRR14241199-combined-coverage-1.gp
│   ├── SRR14241199-combined-coverage-1.png
│   ├── SRR14241199-combined-depth.gp
│   ├── SRR14241199-combined-depth.png
│   ├── SRR14241199-combined-read-perc.gp
│   ├── SRR14241199-combined-read-perc.png
│   ├── SRR14241199-combined-reads.gp
│   ├── SRR14241199-combined-reads.png
│   ├── SRR14241199-combined-tcoord.gp
│   ├── SRR14241199-combined-tcoord.png
│   ├── SRR14241199-combined-tdepth.gp
│   ├── SRR14241199-combined-tdepth.png
│   ├── SRR14241199-heat-amp-1.gp
│   ├── SRR14241199-heat-amp-1.png
│   ├── SRR14241199-heat-coverage-1-1.gp
│   ├── SRR14241199-heat-coverage-1-1.png
│   ├── SRR14241199-heat-read-perc-1.gp
│   ├── SRR14241199-heat-read-perc-1.png
│   ├── SRR14241199-heat-read-perc-log-1.gp
│   ├── SRR14241199-heat-read-perc-log-1.png
│   ├── SRR14241199-heat-reads-1.gp
│   ├── SRR14241199-heat-reads-1.png
│   ├── SRR14241199-SRR14241199.primertrim.sorted-amp.gp
│   ├── SRR14241199-SRR14241199.primertrim.sorted-amp.png
│   ├── SRR14241199-SRR14241199.primertrim.sorted-cov.gp
│   ├── SRR14241199-SRR14241199.primertrim.sorted-cov.png
│   ├── SRR14241199-SRR14241199.primertrim.sorted-reads.gp
│   ├── SRR14241199-SRR14241199.primertrim.sorted-reads.png
│   ├── SRR14241199-SRR14241199.primertrim.sorted-tcoord.gp
│   ├── SRR14241199-SRR14241199.primertrim.sorted-tcoord.png
│   ├── SRR14241199-SRR14241199.primertrim.sorted-tdepth.gp
│   ├── SRR14241199-SRR14241199.primertrim.sorted-tdepth.png
│   ├── SRR14241199-SRR14241199.primertrim.sorted-tsize.gp
│   └── SRR14241199-SRR14241199.primertrim.sorted-tsize.png
├── seqyclean
│   ├── Combined_SummaryStatistics.tsv
│   ├── ERR5181310_clean_PE1.fastq.gz
│   ├── ERR5181310_clean_PE2.fastq.gz
│   ├── ERR5181310_clean_SummaryStatistics.tsv
│   ├── ERR5181360_clean_PE1.fastq.gz
│   ├── ERR5181360_clean_PE2.fastq.gz
│   ├── ERR5181360_clean_SummaryStatistics.tsv
│   ├── ERR5405022_clean_PE1.fastq.gz
│   ├── ERR5405022_clean_PE2.fastq.gz
│   ├── ERR5405022_clean_SummaryStatistics.tsv
│   ├── ERR5556343_clean_PE1.fastq.gz
│   ├── ERR5556343_clean_PE2.fastq.gz
│   ├── ERR5556343_clean_SummaryStatistics.tsv
│   ├── ERR5691945_clean_PE1.fastq.gz
│   ├── ERR5691945_clean_PE2.fastq.gz
│   ├── ERR5691945_clean_SummaryStatistics.tsv
│   ├── ERR5743043_clean_PE1.fastq.gz
│   ├── ERR5743043_clean_PE2.fastq.gz
│   ├── ERR5743043_clean_SummaryStatistics.tsv
│   ├── ERR5743893_clean_PE1.fastq.gz
│   ├── ERR5743893_clean_PE2.fastq.gz
│   ├── ERR5743893_clean_SummaryStatistics.tsv
│   ├── ERR5749609_clean_PE1.fastq.gz
│   ├── ERR5749609_clean_PE2.fastq.gz
│   ├── ERR5749609_clean_SummaryStatistics.tsv
│   ├── ERR5771988_clean_PE1.fastq.gz
│   ├── ERR5771988_clean_PE2.fastq.gz
│   ├── ERR5771988_clean_SummaryStatistics.tsv
│   ├── SRR13422799_clean_PE1.fastq.gz
│   ├── SRR13422799_clean_PE2.fastq.gz
│   ├── SRR13422799_clean_SummaryStatistics.tsv
│   ├── SRR13422802_clean_PE1.fastq.gz
│   ├── SRR13422802_clean_PE2.fastq.gz
│   ├── SRR13422802_clean_SummaryStatistics.tsv
│   ├── SRR13500958_clean_PE1.fastq.gz
│   ├── SRR13500958_clean_PE2.fastq.gz
│   ├── SRR13500958_clean_SummaryStatistics.tsv
│   ├── SRR13511054_clean_PE1.fastq.gz
│   ├── SRR13511054_clean_PE2.fastq.gz
│   ├── SRR13511054_clean_SummaryStatistics.tsv
│   ├── SRR13603869_clean_PE1.fastq.gz
│   ├── SRR13603869_clean_PE2.fastq.gz
│   ├── SRR13603869_clean_SummaryStatistics.tsv
│   ├── SRR13606410_clean_PE1.fastq.gz
│   ├── SRR13606410_clean_PE2.fastq.gz
│   ├── SRR13606410_clean_SummaryStatistics.tsv
│   ├── SRR14241199_clean_PE1.fastq.gz
│   ├── SRR14241199_clean_PE2.fastq.gz
│   └── SRR14241199_clean_SummaryStatistics.tsv
├── snp-dists
│   └── snp-dists.txt
├── software_versions.yml
└── vadr
    ├── combined.fasta
    ├── trimmed.fasta
    ├── vadr.vadr.alc
    ├── vadr.vadr.alt
    ├── vadr.vadr.alt.list
    ├── vadr.vadr.cmd
    ├── vadr.vadr.dcr
    ├── vadr.vadr.fail.fa
    ├── vadr.vadr.fail.list
    ├── vadr.vadr.fail.tbl
    ├── vadr.vadr.filelist
    ├── vadr.vadr.ftr
    ├── vadr.vadr.log
    ├── vadr.vadr.mdl
    ├── vadr.vadr.pass.fa
    ├── vadr.vadr.pass.list
    ├── vadr.vadr.pass.tbl
    ├── vadr.vadr.rpn
    ├── vadr.vadr.sda
    ├── vadr.vadr.seqstat
    ├── vadr.vadr.sgm
    ├── vadr.vadr.sqa
    └── vadr.vadr.sqc

Final files

There are two main files that summarize the information from this workflow. One is a csv file with the key result from each process, and one is the multiqc report. The default location for these files is cecret/cecret_results.csv and cecret/multiqc/multiqc_report.html. If using an alternative destination, set params.outdir to your preferred destination (see: instructions on how to adjust parameters).

Summary csv file

There are summary files for each run found at cecret/cecret_results.csv and cecret/cecret_results.txt. These two files are exactly the same except for the delimiter used to separate the columns.

An example file run with the default values for some SARS-CoV-2 fastq files SRR13957125, SRR13957170, and SRR13957177 are below.

sample_id sample pangolin_lineage nextclade_clade vadr_p/f fasta_line fastqc_raw_reads_1 fastqc_raw_reads_2 num_N num_total seqyclean_PairsKept seqyclean_Perc_Kept num_pos_100X aci_num_failed_amplicons insert_size_after_trimming ivar_num_variants_identified bcftools_variants_identified samtools_meandepth_after_trimming samtools_per_1X_coverage_after_trimming vadr_model vadr_alerts nextclade_clade_who nextclade_qc_overallscore nextclade_qc_overallstatus pangolin_conflict pangolin_ambiguity_score pangolin_scorpio_call pangolin_scorpio_support pangolin_scorpio_conflict pangolin_scorpio_notes pangolin_version pangolin_pangolin_version pangolin_scorpio_version pangolin_constellation_version pangolin_is_designated pangolin_qc_status pangolin_qc_notes pangolin_note pangocollapse_lineage pangocollapse_Lineage_full pangocollapse_Lineage_expanded pangocollapse_Lineage_family freyja_summarized Cecret version seqyclean bwa ivar ivar consensus
SRR13957125 SRR13957125 B.1.429 21C PASS SRR13957125 670879.0 670879.0 667.0 29875.0 576244.0 85.8939 29200.0 2.0 199.0 27.0 27.0 5401.08 99.6522 NC_045512 - Epsilon 12.958697 good 0.0 Epsilon (B.1.429-like) 1.0 0.0 scorpio call: Alt alleles 14; Ref alleles 0; Amb alleles 0; Oth alleles 0 PUSHER-v1.23.1 4.3.1 0.3.19 v0.1.12 False pass Ambiguous content: 4% Usher placements: B.1.429(1/1) B.1.429 B.1.429 B.1.429 B [('Epsilon' 0.9990499999965543)] v3.10.20231226 seqyclean : Version: 1.10.09 (2018-10-16) bwa : Version: 0.7.17-r1188 ivar : iVar version 1.4.2 iVar version 1.4.2
SRR13957170 SRR13957170 Unassigned SRR13957170 2287.0 2287.0 25545.0 25545.0 176.0 7.69567 0.0 99.0 160.0 0.0 6.0 0.182791 6.91235 PUSHER-v1.23.1 4.3.1 0.3.19 v0.1.12 False fail Failed to map Unassigned Unassigned Unassigned Unassigned v3.10.20231226 seqyclean : Version: 1.10.09 (2018-10-16) bwa : Version: 0.7.17-r1188 ivar : iVar version 1.4.2 iVar version 1.4.2
SRR13957177 SRR13957177 B.1.1.7 20I PASS SRR13957177 902426.0 902426.0 776.0 29787.0 837318.0 92.7852 29019.0 2.0 207.3 39.0 41.0 7621.74 99.8495 NC_045512 - Alpha 5.885816 good 0.0 Alpha (B.1.1.7-like) 0.96 0.04 scorpio call: Alt alleles 22; Ref alleles 1; Amb alleles 0; Oth alleles 0 PUSHER-v1.23.1 4.3.1 0.3.19 v0.1.12 False pass Ambiguous content: 4% Usher placements: B.1.1.7(1/1) B.1.1.7 B.1.1.7 B.1.1.7 B.1.1.7 [('Alpha' 0.999009112161133)] v3.10.20231226 seqyclean : Version: 1.10.09 (2018-10-16) bwa : Version: 0.7.17-r1188 ivar : iVar version 1.4.2 iVar version 1.4.2

MultiQC report

The multiqc report aggregates data across your samples into one file. Open the 'cecret/multiqc/multiqc_report.html' file with your favored browser. There tables and graphs are generated for 'General Statistics', 'Samtools stats', 'Samtools flagstats', 'FastQC', 'SeqyClean', 'Fastp', 'Pangolin', and 'Kraken2'. There are also added custom sections for many additional analysis including Freyja.

Example fastqc graphs

fastqc_per_base_sequence_quality_plot fastqc_sequence_counts_plot

Example kraken2 graph

Example pangolin graph

Screenshot 2025-06-24 at 11 23 45 AM

Nextflow output

Nextflow will also produce files. These files are more about the resources that the system used than about analysis of the data. They are found in {params.outdir}/pipeline_info/.

nf-*-reports.tsv

If using Nextflow Tower or Seqera Platform, these are the files that appear in the reports section of the UI.

Currently, the summary file for the workflow (cecret_results.*) and the multiqc report (multiqc_report.html) are included. More may be added once UPHL gains access to this resource.

report-*-*.html

This is a report of the workflow that may be useful in determining computational resource use. This includes the command that was used, the directories and other storage locations that were used, and information about the processes being run.

timeline-*-*.html

Visually shows the timeline for each process given the computational environment.

work

A directory for all the temporary files used in the analysis. Users can generally delete this directory when the workflow is complete because 1) it generally is not needed if everything is run successfully and 2) because it is VERY large. Users will not be able to use nextflow -resume if this directory is deleted.

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