Differential Analysis - TealFurnholm/Strain-Level_Metatranscriptome_Analysis GitHub Wiki
!- under construction as of 2-16-21 - need to update the R script to take current file names
For Comparative Sample Analysis:
- fill in the design.matrix = the sample names and which group they belong (eg. control, disease-only, drug-only, disease+drug)
wget https://github.com/TealFurnholm/Metatranscriptome/blob/master/design.matrix
- fill in the contrast.matrix = each group and how you want them compared (eg. disease+drug vs drug-only)
wget https://github.com/TealFurnholm/Metatranscriptome/blob/master/contrast.matrix
- run the R analysis, described here: https://github.com/TealFurnholm/Metatranscriptome/blob/master/Metatranscriptome_Limma_R_Analysis.ipynb