Outline SmallRNA Commands - SabaLab/RNASeq_Scripts GitHub Wiki

  • Generate novel MiRNA Features
    1. run Genome Alignment
    2. Convert to format for MirDeep
    3. Run MirDeep on Parental Strains
    4. Parse/Merge MirDeep features
  • Generate novel SnoRNA Features
    1. Run SnoSeeker on each parental strain specific genome
    2. Parse/Merge SnoSeeker output
  • Trim Reads
  • Align rRNA
  • Align miRNA
  • Align snoRNA
  • Align miscSmallRNA
  • Align miscLargeRNA
  • Parse features from each fraction