RNA seq - SMRUCC/GCModeller GitHub Wiki
Module AssemblyName: file:///G:/GCModeller/manual/bin/RNA-seq.exe Root namespace: RNA_seq.CLI
All of the command that available in this program has been list below:
Function API | Info |
---|---|
/Associate.GI | |
/Clustering | |
/Co.Vector | |
/Contacts.Ref | |
/Data.Frame | Generates the data input for the DESeq2 R package. |
/DataFrame.RPKMs | Merges the RPKM csv data files. |
/DEGs | |
/DEGs.UpDown | |
/DESeq2 | |
/DOOR.Corrects | |
/Export.Megan.BIOM | |
/Export.SAM.Maps | |
/Export.SSU.Refs | |
/Export.SSU.Refs.Batch | |
/Format.GI | |
/fq2fa | |
/gast | |
/gast.stat.names | |
/Group.n | |
/HT-seq | Count raw reads for DESeq2 analysis. |
/Imports.gast.Refs.NCBI_nt | |
/log2.selects | |
/PCC | |
/Rank.Statics | |
/RPKM | |
/RPKM.Log2 | |
/sid.map | |
/SPCC | |
/Stat.Changes | |
/Statics.Labels | |
/WGCNA | Generates the cytoscape network model from WGCNA analysis. |
Prototype: RNA_seq.CLI::Int32 AssociateGI(Microsoft.VisualBasic.CommandLine.CommandLine)
Information:
Usage: G:\GCModeller\manual\bin\RNA-seq.exe /Associate.GI /in <list.Csv.DIR> /gi <nt.gi.csv> [/out <out.DIR>]
Example: RNA-seq /Associate.GI
Prototype: RNA_seq.CLI::Int32 Clustering(Microsoft.VisualBasic.CommandLine.CommandLine)
Information:
Usage: G:\GCModeller\manual\bin\RNA-seq.exe /Clustering /in <fq> /kmax <int> [/out <out.Csv>]
Example: RNA-seq /Clustering
Prototype: RNA_seq.CLI::Int32 CorrelatesVector(Microsoft.VisualBasic.CommandLine.CommandLine)
Information:
Usage: G:\GCModeller\manual\bin\RNA-seq.exe /Co.Vector /in <co.Csv/DIR> [/min 0.01 /max 0.05 /out <out.csv>]
Example: RNA-seq /Co.Vector
Prototype: RNA_seq.CLI::Int32 Contacts(Microsoft.VisualBasic.CommandLine.CommandLine)
Information:
Usage: G:\GCModeller\manual\bin\RNA-seq.exe /Contacts.Ref /in <in.fasta> /maps <maps.sam> [/out <out.DIR>]
Example: RNA-seq /Contacts.Ref
Prototype: RNA_seq.CLI::Int32 Df(Microsoft.VisualBasic.CommandLine.CommandLine)
Information: Generates the data input for the DESeq2 R package.
Usage: G:\GCModeller\manual\bin\RNA-seq.exe /Data.Frame /in <in.DIR> /ptt <genome.ptt> [/out out.csv]
Example: RNA-seq /Data.Frame
Parameters information:
/in
Description: A directory location which it contains the Ht-Seq raw count text files.
Example: /in ""
Prototype: RNA_seq.CLI::Int32 MergeRPKMs(Microsoft.VisualBasic.CommandLine.CommandLine)
Information: Merges the RPKM csv data files.
Usage: G:\GCModeller\manual\bin\RNA-seq.exe /DataFrame.RPKMs /in <in.DIR> [/trim /out <out.csv>]
Example: RNA-seq /DataFrame.RPKMs
Prototype: RNA_seq.CLI::Int32 DEGs(Microsoft.VisualBasic.CommandLine.CommandLine)
Information:
Usage: G:\GCModeller\manual\bin\RNA-seq.exe /DEGs /in <diff.csv> [/out <degs.csv> /log_fold 2]
Example: RNA-seq /DEGs
Prototype: RNA_seq.CLI::Int32 DEGsUpDown(Microsoft.VisualBasic.CommandLine.CommandLine)
Information:
Usage: G:\GCModeller\manual\bin\RNA-seq.exe /DEGs.UpDown /in <diff.csv> /sample.table <sampleTable.Csv> [/out <outDIR>]
Example: RNA-seq /DEGs.UpDown
Prototype: RNA_seq.CLI::Int32 DESeq2(Microsoft.VisualBasic.CommandLine.CommandLine)
Information:
Usage: G:\GCModeller\manual\bin\RNA-seq.exe /DESeq2 /sample.table <sampleTable.csv> /raw <raw.txt.DIR> /ptt <genome.ptt> [/design <design, default: ~condition>]
Example: RNA-seq /DESeq2
Prototype: RNA_seq.CLI::Int32 DOORCorrects(Microsoft.VisualBasic.CommandLine.CommandLine)
Information:
Usage: G:\GCModeller\manual\bin\RNA-seq.exe /DOOR.Corrects /DOOR <genome.opr> /pcc <pcc.dat> [/out <out.opr> /pcc-cut <0.45>]
Example: RNA-seq /DOOR.Corrects
Prototype: RNA_seq.CLI::Int32 ExportToMegan(Microsoft.VisualBasic.CommandLine.CommandLine)
Information:
Usage: G:\GCModeller\manual\bin\RNA-seq.exe /Export.Megan.BIOM /in <relative.table.csv> [/out <out.json.biom>]
Example: RNA-seq /Export.Megan.BIOM
Prototype: RNA_seq.CLI::Int32 ExportSAMMaps(Microsoft.VisualBasic.CommandLine.CommandLine)
Information:
Usage: G:\GCModeller\manual\bin\RNA-seq.exe /Export.SAM.Maps /in <in.sam> [/contigs <NNNN.contig.Csv> /raw <ref.fasta> /out <out.Csv>]
Example: RNA-seq /Export.SAM.Maps
Prototype: RNA_seq.CLI::Int32 ExportSSURefs(Microsoft.VisualBasic.CommandLine.CommandLine)
Information:
Usage: G:\GCModeller\manual\bin\RNA-seq.exe /Export.SSU.Refs /in <ssu.fasta> [/out <out.DIR> /no-suffix]
Example: RNA-seq /Export.SSU.Refs
Prototype: RNA_seq.CLI::Int32 ExportSSUBatch(Microsoft.VisualBasic.CommandLine.CommandLine)
Information:
Usage: G:\GCModeller\manual\bin\RNA-seq.exe /Export.SSU.Refs /in <ssu.fasta.DIR> [/out <out.DIR>]
Example: RNA-seq /Export.SSU.Refs.Batch
Prototype: RNA_seq.CLI::Int32 FormatGI(Microsoft.VisualBasic.CommandLine.CommandLine)
Information:
Usage: G:\GCModeller\manual\bin\RNA-seq.exe /Format.GI /in <txt> /gi <regex> /format <gi|{gi}> /out <out.txt>
Example: RNA-seq /Format.GI
Prototype: RNA_seq.CLI::Int32 Fq2fa(Microsoft.VisualBasic.CommandLine.CommandLine)
Information:
Usage: G:\GCModeller\manual\bin\RNA-seq.exe /fq2fa /in <fastaq> [/out <fasta>]
Example: RNA-seq /fq2fa
Prototype: RNA_seq.CLI::Int32 gastInvoke(Microsoft.VisualBasic.CommandLine.CommandLine)
Information:
Usage: G:\GCModeller\manual\bin\RNA-seq.exe
Example: RNA-seq /gast
Prototype: RNA_seq.CLI::Int32 StateNames(Microsoft.VisualBasic.CommandLine.CommandLine)
Information:
Usage: G:\GCModeller\manual\bin\RNA-seq.exe /gast.stat.names /in <*.names> /gast <gast.out> [/out <out.Csv>]
Example: RNA-seq /gast.stat.names
Prototype: RNA_seq.CLI::Int32 GroupN(Microsoft.VisualBasic.CommandLine.CommandLine)
Information:
Usage: G:\GCModeller\manual\bin\RNA-seq.exe /Group.n /in <dataset.csv> [/locus_map <locus> /out <out.csv>]
Example: RNA-seq /Group.n
Prototype: RNA_seq.CLI::Int32 HTSeqCount(Microsoft.VisualBasic.CommandLine.CommandLine)
Information: Count raw reads for DESeq2 analysis.
Usage: G:\GCModeller\manual\bin\RNA-seq.exe /Ht-seq /in <in.sam> /gff <genome.gff> [/out <out.txt> /mode <union, intersection_strict, intersection_nonempty; default:intersection_nonempty> /rpkm /feature <CDS>]
Example: RNA-seq /HT-seq
Parameters information:
[/Mode]
Description: The value of this parameter specific the counter of the function will be used, the available counter values are: union, intersection_strict and intersection_nonempty
Example: /Mode ""
[/feature]
Description: [NOTE: value is case sensitive!!!] Value of the gff features can be one of the: tRNA, CDS, exon, gene, tmRNA, rRNA, region
Example: /feature ""
Prototype: RNA_seq.CLI::Int32 ImportsRefFromNt(Microsoft.VisualBasic.CommandLine.CommandLine)
Information:
Usage: G:\GCModeller\manual\bin\RNA-seq.exe /Imports.gast.Refs.NCBI_nt /in <in.nt> /gi2taxid <dmp/txt/bin> /taxonomy <nodes/names.dmp_DIR> [/out <out.fasta>]
Example: RNA-seq /Imports.gast.Refs.NCBI_nt
Prototype: RNA_seq.CLI::Int32 Log2Selects(Microsoft.VisualBasic.CommandLine.CommandLine)
Information:
Usage: G:\GCModeller\manual\bin\RNA-seq.exe /log2.selects /log2 <rpkm.log2.csv> /data <dataset.csv> [/locus_map <locus> /factor 1 /out <out.dataset.csv>]
Example: RNA-seq /log2.selects
Prototype: RNA_seq.CLI::Int32 PCC(Microsoft.VisualBasic.CommandLine.CommandLine)
Information:
Usage: G:\GCModeller\manual\bin\RNA-seq.exe /PCC /expr <expr.matrix.csv> [/out <out.dat>]
Example: RNA-seq /PCC
Prototype: RNA_seq.CLI::Int32 RankStatics(Microsoft.VisualBasic.CommandLine.CommandLine)
Information:
Usage: G:\GCModeller\manual\bin\RNA-seq.exe /Rank.Statics /in <relative.table.csv> [/out <EXPORT_DIR>]
Example: RNA-seq /Rank.Statics
Prototype: RNA_seq.CLI::Int32 RPKM(Microsoft.VisualBasic.CommandLine.CommandLine)
Information:
Usage: G:\GCModeller\manual\bin\RNA-seq.exe /RPKM /raw <raw_count.txt> /gff <genome.gff> [/out <expr.out.csv>]
Example: RNA-seq /RPKM
Prototype: RNA_seq.CLI::Int32 Log2(Microsoft.VisualBasic.CommandLine.CommandLine)
Information:
Usage: G:\GCModeller\manual\bin\RNA-seq.exe /RPKM.Log2 /in <RPKM.csv> /cond <conditions> [/out <out.csv>]
Example: RNA-seq /RPKM.Log2
Parameters information:
/cond
Description: Syntax format as: <experiment1>/<experiment2>|<experiment3>/<experiment4>|.....
Example: /cond "colR1/xcb1|colR2/xcb2"
Prototype: RNA_seq.CLI::Int32 sIdMapping(Microsoft.VisualBasic.CommandLine.CommandLine)
Information:
Usage: G:\GCModeller\manual\bin\RNA-seq.exe /sid.map /gff <genome.gff> /raw <htseq-count.txt> [/out <out.txt>]
Example: RNA-seq /sid.map
Prototype: RNA_seq.CLI::Int32 SPCC(Microsoft.VisualBasic.CommandLine.CommandLine)
Information:
Usage: G:\GCModeller\manual\bin\RNA-seq.exe /SPCC /expr <expr.matrix.csv> [/out <out.dat>]
Example: RNA-seq /SPCC
Prototype: RNA_seq.CLI::Int32 StatChanges(Microsoft.VisualBasic.CommandLine.CommandLine)
Information:
Usage: G:\GCModeller\manual\bin\RNA-seq.exe /Stat.Changes /deseq <deseq.result.csv> /sample <sampletable.csv> [/out <out.csv> /levels <1000> /diff <0.5>]
Example: RNA-seq /Stat.Changes
Prototype: RNA_seq.CLI::Int32 MergeLabels(Microsoft.VisualBasic.CommandLine.CommandLine)
Information:
Usage: G:\GCModeller\manual\bin\RNA-seq.exe /Statics.Labels /in <in.csv> [/label <Label> /name <Name> /value <value> /out <out.csv>]
Example: RNA-seq /Statics.Labels
Prototype: RNA_seq.CLI::Int32 FromWGCNA(Microsoft.VisualBasic.CommandLine.CommandLine)
Information: Generates the cytoscape network model from WGCNA analysis.
Usage: G:\GCModeller\manual\bin\RNA-seq.exe /WGCNA /data <dataExpr.csv> /anno <annotations.csv> [/out <DIR.Out> /mods <color.list> /from.DESeq /id.map <GeneId>]
Example: RNA-seq /WGCNA
Parameters information:
/data
Description: A sets of RNA-seq RPKM expression data sets, the first row in the csv table should be the experiments or conditions, and first column in the table should be the id of the genes and each cell in the table should be the RPKM expression value of each gene in each condition.
The data format of the table it would be like:
GeneId, condi1, cond12, condi3, ....
locus1, x, xx, x,
locus2, y, yy, yyy,
locus3, ,z, zz, zzz,
......
xyz Is the RPKM of the genes
Example: /data ""
/anno
Description: A table of the gene name annotation, the table should be in formats of
Id, gene_symbol
locus1, geneName
locus2, geneName
....
Example: /anno ""
[/mods]
Description: Each color in this parameter value is stands for a co expression module, and this parameter controls of the module output filtering, using | character as the seperator for each module color.
Example: /mods ""
[/out]
Description: Export directory of the WGCNA data, if this parameter value is not presents in the arguments, then the current work directory will be used.
Example: /out ""
[/From.Deseq]
Description: Is the /data matrix if comes from the DESeq analysis result output?
If is true, then the expression value will be extract from the original matrix file and save a new file named DESeq.dataExpr0.Csv in the out directory,
and last using this extracted data as the source of the WGCNA R script.
Example: /From.Deseq ""