2017 Oyster Larvae Trials - RobertsLab/project-pacific.oyster-larvae GitHub Wiki
This experiment was conducted at the Taylor Shellfish Hatchery from 6/27/17 to 7/14/17 by Rhonda Elliott to test differences in larval survival in different systems throughout the hatchery. Oyster larvae production has been poor all season at the hatchery with particularly poor survival in the production high density flow through system. We have seen better survival in larval cohorts when reared in different parts of the hatchery. The goal of this experiment is to investigate those differences to better understand what is happening by pumping water from the production area to different parts of the hatchery. If there is a problem associated with the water in the production area we should see those same negative effects on larval survival when we pump that water to different locations in the hatchery. In a previous trial, we saw increased growth and survival when we heated the water to 28C on Day 3 in the New Greenhouse system. In this experiment we will increase the temperature to 28C in the Production room to see if we see the same results as in the New Greenhouse. We sampled for proteomics, microbiomes, and morphology.
Notebook: https://ellior2.github.io/Oysterlarvaetrials2017/
Descriptions of location/water systems:
Production area: The largest larvae culture area in the hatchery which is where we grow all of our production oysters. This system has 48 conicals (270L). This water has been filtered down to 25um with a drum filter where it then enters our large 12,000L treatment tanks. This treatment tank has a loop where the water is treated with ozone and protein skimming and enters back into the tank. Before the water is sent to the production area it is treated with UV. This water is then filtered down to 5um and pumped into a large round reservoir in the production room where it is heated to 23C using a heat recovery system. The heated seawater is pumped into a header where it is mixed with algae before being pumped into the flow through conicals with larvae. High density microalgae to this system is supplied by Bag System 1 and 2 at the hatchery.
New Greenhouse: This system has 10 conicals (270L). This water does not receive ozone/protein skimming/UV treated water. The water is fed from the main hatchery and is heated to 23C. The seawater is filtered down to 5um. This system receives microalgae from Bag System 3 located in the greenhouse.
Duck room: This system has 6 conicals (350L). It receives water directly from the treatment tanks, is heated to 26C and is filtered down to 10um. By the time the water reaches the conicals it is about ~24C. This system receives the same food as the production area from Bag System 1 and 2.
Experimental Design
Production Room:
Conicals 41 and 42: Production water heated to 28C at Day 3
Conicals 45 and 46: Treatment tank water
Conicals 47 and 48: Production water (Control)
New Greenhouse:
Conicals 1 and 2: Production room water
Conicals 3 and 4: New Greenhouse water (control)
Duck Room:
Conicals 1 and 2: Production room water
Conicals 6 and 7: Duck room water (control)
Timeline:
6/27/17 Production spawn of PT22. 50 million eggs were put into each of the 14 conicals with a 28um banjo and water flow rate of 0.2gpm.
6/28/17 Larvae were fed up to 50,000 cells/ml incoming in the morning.
6/29/17 Dropped conicals and sampled for proteomics then did a D-hinge count to estimate hatching rate using coulter counter. I used the lowest D-hinge count to determine how many larvae to put back in each conical. In this case the conical with the lowest hatching rate was New Greenhouse- Production water Conical 2 with 23,255,000 larvae. Thus I put 23,255,000 larvae back from each of the conicals regardless of hatching rate. This was to avoid any density related factors that may arise during larval rearing period.
6/30/17 Moved conicals 41 and 42 to 28C.
7/1/17 Microbiome sampling, dropped conicals, proteomic sampling, put Hobo loggers in
7/02/17 Measurements
7/03/17 Dropped conicals, proteomics
7/04/17 Microbiome sampling, measurements
7/05/17 Screening day, proteomics, histology, PAXgene
7/06/17 Microbiome sampling, measurements
7/07/17 Drop conicals, proteomics
7/09/17 Screening day, proteomics, measurements
7/11/17 Drop conicals, proteomics, measurements
7/12/17 Microbiome sampling
7/13/17 Quantify larvae, proteomics, measurements
7/14/17 Histology samples and end of experiment
Here is temperature data from Hobo loggers starting from 7/1/17.
