Structural Variant Calling data set (Sequel System 6.0 release) - PacificBiosciences/DevNet GitHub Wiki

SAMPLE

GIAB HG002 extracted DNA

METHODS

Shearing 15 kb with g-TUBE
Library prep SMRTbell Express
Size selection >15 kb with BluePippin
Sequencing Sequel System with binding kit 3.0 (101-500-400) and chemistry 3.0 (101-427-800)
Run time 10 hrs per SMRT Cell
Reference GCA_000001405.15_GRCh38_no_alt_analysis_set
Variant Calling Structural Variant Calling analysis in SMRT Link 6.0

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URLs and md5 checksums are listed in URLs.txt