Microbial Multiplexing Data Set 48 plex: PacBio Sequel II System, Chemistry v2.0, SMRT Link v8.0 Analysis - PacificBiosciences/DevNet GitHub Wiki
SAMPLE
15 microbial isolates acquired from ATCC
- Bacillus cereus 971
- Bacillus subtilis W23
- Burkholderia cepacia
- Enterococcus faecalis OG1RF
- Escherichia coli K12
- Escherichia coli W
- Helicobacter pylori J99
- Klebsiella pneumoniae
- Listeria monocytogenes*
- Methanocorpusculum labreanum Z
- Neisseria meningitidis FAM18
- Rhodopseudomonas palustris
- Staphylococcus aureus*
- Staphylococcus aureus subsp. aureus
- Treponema denticola A
*Sequencing results differ significantly from published references of the named strains, indicating that these microbes are most likely mislabeled.
METHODS
- Microbial multiplexing protocol (https://www.pacb.com/wp-content/uploads/Procedure-Checklist-Preparing-Multiplexed-Microbial-Libraries-Using-SMRTbell-Express-Template-Prep-Kit-2.0.pdf)
- Library prep: SMRTbell Express Template Prep Kit 2.0
- Sequencing: Sequel II System binding 2.0 kit (101-789-500) and Sequel II Sequencing 2.0 kit (101-820-200)
- Run time: 2 hour pre-extension; 15 hour movie
- Analysis Application: SMRT Link v8.0 Microbial Assembly
DOWNLOAD
Download Data: https://downloads.pacbcloud.com/public/dataset/MicrobialMultiplexing_48plex/