HG002 Structural Variant Analysis with CLR data - PacificBiosciences/DevNet GitHub Wiki

SAMPLE

GIAB HG002 extracted DNA

METHODS

• Shearing 75 kb with Megaruptor
• Library prep SMRTbell Express 2.0
• Size selection >30 kb with BluePippin
• Sequencing Sequel II System with "Early Access" binding kit (101-490-800) and chemistry (101-490-900)
• Run time 15 hrs per SMRT Cell
• Reference hs37d5 (GRCh37 with decoy)
• Variant Calling Structural Variant Calling analysis in SMRT Link 6.1

DOWNLOAD

URLs and md5 checksums are listed in URLs.txt

Download Data: https://downloads.pacbcloud.com/public/dataset/SV-HG002-CLR/

Download from China: https://downloads-ap.pacbcloud.com/public/dataset/SV-HG002-CLR/