E coli K12 MG1655 Resequencing - PacificBiosciences/DevNet GitHub Wiki
Instrument: PacBio RS
Chemistry: C2
Enzyme: C2
This Escherichia coli resequencing dataset showcases PacBio®'s extremely long-read, single molecule data with a sample of the E. coli K12 MG1655 strain.
Data consist of raw, unfiltered base files as well as data analysis with SMRT® Pipe, including filtered reads in FASTQ format, mapped reads in SAM/BAM, consensus calls and variants in VCF format.
Key metrics for sample data on v1.3.0 with C2 chemistry:
- 1 SMRT Cell, one 90 min. movie to maximize read length
- Consensus accuracy: 99.99% (Q40)
- Mean mapped read length: 3,549 bp
- 95th percentile mapped read length: 9,009 bp
- Coverage: 19.96x
- Mapped bases: 95.26 Mbp
Instrument Output (Primary Analysis) Data
- E. coli K12 MG1655 Raw Reads HDF 1.15 GB md5