E coli K12 MG1655 Methylome - PacificBiosciences/DevNet GitHub Wiki
Instrument: PacBio RS
Chemistry: C2
Enzyme: C2
Summary
These data are intended for use with the Technical Note, Detecting DNA Base Modifications: SMRT® Analysis of Microbial Methylomes.
The examples include:
- The appropriate reference sequence FASTA file
- Five SMRT Cells of native E. coli data (Native Raw Reads HDF) with an analysis result comparing to the in silico control (Native Analysis)
- Six SMRT Cells of whole genome amplified data (WGA Raw Reads HDF) aligned to the appropriate reference
- An analysis of the native sequence data using the WGA as an amplified control (Native vs WGA Analysis)
Related Topics
Technical Note: Detecting DNA Base Modifications: SMRT® Analysis of Microbial Methylomes.
Download Datasets
Primary Analysis (Instrument Output) Data
- Native raw reads in HDF5 format 11.7 GB md5
- WGA raw reads in HDF5 format 12.3 GB md5
E coli K12 Reference Genome
Secondary Analysis Data
- Native Analysis 5.9 GB md5
- WGA Analysis 5.6 GB md5
- Native vs WGA Analysis 6.0 GB md5
- SMRT Analysis 1.3.3 Methylome Anlysis 5.5 GB
Tertiary Analysis Data
- The REBASE entry of predicted Modification sites for this organism is here.