16S Data Set Sequel II System 2.0 Release - PacificBiosciences/DevNet GitHub Wiki

SAMPLE

20 Strain Staggered Mix Genomic Material (ATCC® MSA-1003™) https://www.atcc.org/products/all/MSA-1003.aspx

METHODS

• 16S protocol with Barcoded Primers (https://www.pacb.com/wp-content/uploads/Procedure-Checklist-Full-Length-16S-Amplification-SMRTbell-Library-Preparation-and-Sequencing.pdf)
• Library prep: SMRTbell Express Template Prep Kit 2.0
• Sequencing: Sequel II System binding kit (101-820-500) and chemistry (101-826-100)
• Run time: 0.5 hour pre-extension; 10 hour movie
• CCS Analysis: SMRT Link v10.0 Circular Consensus Sequencing Application (ccs 5.0.0)

DOWNLOAD

Complete 192 plex dataset: http://downloads.pacbcloud.com/public/dataset/atcc_msa/16S_192plex_HiFi.fastq.tar.gz

Example of reads from a single sample: http://downloads.pacbcloud.com/public/dataset/atcc_msa/demultiplex.16S_For_bc1008--16S_Rev_bc1065.hifi_reads.fastq