Wound Healing Tool - MontpellierRessourcesImagerie/imagej_macros_and_scripts Wiki

The MRI Wound Healing Tool can be used to analyze scratch assays. It measures the area of a wound in a cellular tissue on a stack of images representing a time-series. An example image can be found here. An example image for the find-edges method (see below) can be found here.

Getting Started

To install the tools, save the file MRI_Wound_Healing_Tool.ijm under macros/toolsets in your ImageJ installation.

Select the MRI Wound Healing Tool toolset from the >> button of the ImageJ launcher.



By right-clicking on the m button you can open the options dialog.




wound-healing-res01.png wound-healing-res02.png wound-healing-res03.png

See also

Other (third party) software for scratch assay analysis

Publications using this tool

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  2. Touat-Hamici, Z., Weidmann, H., Blum, Y., Proust, C., Durand, H., Iannacci, F., Codoni, V., Gaignard, P., Thérond, P., Civelek, M., Karabina, S.A., Lusis, A.J., Cambien, F., Ninio, E., 2016. Role of lipid phosphate phosphatase 3 in human aortic endothelial cell function. Cardiovasc Res 112, 702–713. https://doi.org/10.1093/cvr/cvw217

  3. Elliott Grant Richards, 2021. The role of decay accelerating factor in the pathogenesis of endometriosis (PhD thesis). Case Western Reserve University.

  4. Bilotta, A.J. et al. (2021) Propionate Enhances Cell Speed and Persistence to Promote Intestinal Epithelial Turnover and Repair, Cellular and Molecular Gastroenterology and Hepatology, 11(4), pp. 1023–1044. doi:10.1016/j.jcmgh.2020.11.011.

  5. Machado, S. et al. (2021) Toxicity in vitro and in Zebrafish Embryonic Development of Gold Nanoparticles Biosynthesized Using Cystoseira Macroalgae Extracts, International Journal of Nanomedicine, Volume 16, pp. 5017–5036. doi:10.2147/IJN.S300674.

  6. Paull, E.O. et al. (2021) A modular master regulator landscape controls cancer transcriptional identity, Cell, 184(2), pp. 334-351.e20. doi:10.1016/j.cell.2020.11.045.

  7. Bownes, L.V. et al. (2021) EZH2 inhibition decreases neuroblastoma proliferation and in vivo tumor growth, PLOS ONE. Edited by J.W. Ramos, 16(3), p. e0246244. doi:10.1371/journal.pone.0246244.

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  12. Erdem, Y.S. et al. (2021) An Image Segmentation Method for Wound Healing Assay Images, Natural and Applied Sciences Journal [Preprint]. doi:10.38061/idunas.853356.

  13. Lee, J.H., Yoo, S.S., Hong, M.J., Choi, J.E., Kang, H., Do, S.K., Lee, W.K., Choi, S.H., Lee, Y.H., Seo, H., et al. (2021). Epigenetic readers and lung cancer: the rs2427964C>T variant of the bromodomain and extraterminal domain gene BRD3 is associated with poorer survival outcome in NSCLC. Mol Oncol 1878-0261.13109.

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  18. Kauanova, S., Urazbayev, A., and Vorobjev, I. (2021). The Frequent Sampling of Wound Scratch Assay Reveals the “Opportunity” Window for Quantitative Evaluation of Cell Motility-Impeding Drugs. Front. Cell Dev. Biol. 9, 64097

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  33. Azar, W.J., Christie, E.L., Mitchell, C., Liu, D.S., Au-Yeung, G., and Bowtell, D.D.L. (2020). Non-canonical IL-6 signaling-mediated activation of YAP regulates cell migration and invasion in ovarian clear cell cancer. Cancer Res canres.3044.2019.

  34. Pucci, M., Gomes Ferreira, I., Orlandani, M., Malagolini, N., Ferracin, M., and Dall’Olio, F. (2020). High Expression of the Sda Synthase B4GALNT2 Associates with Good Prognosis and Attenuates Stemness in Colon Cancer. Cells 9, 948.

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