The tool allows to simulated particle moving away from a center, moving towards a center or particles diffusing around a center.

The source code in git-hub can be found here.

Download the synthetic_tracking_data_generator.ijm and put it into the macros/toolsets folder of your FIJI installation.

Select the "synthetic tracking data generator" toolset from the >> button of the ImageJ launcher.

• The first button opens this help-page.
• The second button creates the moving particles in form of a table containing the ids and coordinates.
• The third button creates a time-series stack showing the moving particles.

Right-click the c-button to open the options for the track creation.

Nr. of Frames

The number of frames in the time-series stack

Image width

The width of the images in pixel.

Image height

The height of the images in pixel.

Nr. of particles

The number of the particles. The number remains constant for all frames.

Center

The coordinates x, y around which the points are arranged and from which they move away.

Initial distance

The mean and stdDev of the initial distance of the points from the center.

speed

The mean and stdDev of the speed of the particles.

angular deviation

The mean and stdDev of the angle of deviation of the particles from their current direction in each step.

Reverse time

Reverses the time after creation of the tracks. This can be used to create particles moving towards the center

Right-click the d-button to open the options for the drawing of the particles.

• spot radius - The radius with which the spots are drawn