Segmentation of mitotic spindles - MontpellierRessourcesImagerie/imagej_macros_and_scripts GitHub Wiki
User manual
Download the script as a .py
file
Open an image where a unique spindle is visible on each frame. Multiple spindles can be bundled in an image if they are assembled as a time sequence.
Open it in ImageJ and adjust the settings in the code:
_stained_channel
: (default: 2) The channel in which your microtubules are stained. (start=1)_rolling_ball_radius
: (default: 50) Same setting as in the Subtract background_min_size
: (default: 90) Minimal area (in number of pixels) covered by an instance of spindle.
You can now run the script, it should provide you with a results table.
When you run the script, a prompt will show up asking you to chose a folder. You should either create a new folder or give the path of an empty one. An ROI will be exported for each frame showing what was segmented. You can edit each individual ROI and press [M] again to add the fixed measurement. You can use the "T" column of the results table, matching the frame index of each spindle to determine which one you can delete.