By Angus Ball

This page is unfinished

Introduction

Welcome to the phylo-rpca tutorial, I've already explained the theory of this in the Beta Diversity page so go there for a refresher. This tutorial also assumes you have Qiime2 set up. Anyways without further ado...

quick reference to how I'll format this document

• This is the step

This is the code you'll run, note the copy button --->

This is the output

• Step 2
  • These are bonus facts that I want to say,
  • or sub steps

installation

wget https://github.com/picrust/picrust2/archive/v2.5.2.tar.gz
tar xvzf  v2.5.2.tar.gz
cd picrust2-2.5.2/
conda env create -f picrust2-env.yaml
conda activate picrust2
pip install --editable .

PS that period is important

running the pipeline

picrust2_pipeline.py -s rep-seqs.fna -i OTU.txt -o picrust2_out_pipeline -p 10 --stratified --verbose