By Angus Ball and Kenzie Chilcott

Introduction

The dead air box is really easy to use and aims to reduce the amount of DNA contamination of environmental sources (sneezing, floating in the air) that can enter your sample. You should use the dead air box when setting up PCR or ethanol precipitations, ect. You are NOT to use the dead air box when doing DNA extractions, the raw quantity of DNA you'd be introducing into the system is too much.

What you will need:

1. Dead air box
2. 10% bleach
3. 70% ethanol

Procedure

1. Spray dead air box with 10 % bleach
2. Wait 10 minutes
3. Spray with 70 % ethanol
4. Cover dead air box and turn on UV light in dead air box for 20 minutes.
5. After 20 min the dead air box is ready for use